细胞培养基的吸收光谱研究  

The Study of Absorption Spectrum for Cell Substrate

作　　者：赵元黎 张风秋[2] 葛向红[2] 姚淑霞[2] 梁二军[2] 

机构地区：[1]中国科学院安徽光学精密机械研究所,安徽合肥230031 [2]郑州大学物理工程学院,河南郑州450052

出　　处：《光谱学与光谱分析》2004年第8期907-910,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金 (1 0 2 0 50 1 3)资助项目

摘　　要：使用日本岛津UV 310 1分光光度计分别测量培养了宫颈癌细胞 (Hela)和鼻咽癌细胞 (CNE)的RP MI 16 4 0培养基和DMEM高糖培养基的紫外吸收光谱 ,分析了培养基中蛋白质的紫外吸收特性。RPMI16 4 0培养基的 2 2 7nm吸收峰在培养细胞生长过程中位移至 2 2 2或 2 18nm ,2 78nm的吸收峰位移至 2 80nm ;而DMEM高糖培养基 2 2 4nm吸收峰在培养细胞生长过程中位移至 2 2 1nm附近 ,2 78nm吸收峰基本没有位移。这些位移说明培养基中芳香族氨基酸中各种氨基酸如色氨酸和酪氨酸的含量有变化。也就是说 ,癌细胞在生长过程中对色氨酸和酪氨酸的消耗不是按等量比的。实验也说明Hela和CNE在生长过程中 ,对RPMI 16 4 0培养基和DMEM高糖培养基中氨基酸的消耗是不同的。The authors collected the absorption spectrum of RPMI 1640 and DMEM substrates that cultivated Hela and CNE by UV-3101 spectrophotometer and analysed the absorbability of proteins in the substrate. The absorption peaks of the RPMI 1 640 culture medium that cultivated cells for different times shifted from 227 to 222 or 218 nm and from 278 to 280 nm respectively; while during growing course of cultivated cells, one of the absorption peaks of DMEM culture medium shifted from 224 nm to one near 221 nm, and the absorption peak 278 nm almost had no shift. All of these shifts show that the content of each amino acid such as tryptophan and casein has already changed. That is, during the growing course of cultivating cancer cells, the tryptophan and casein were not depleted equivalently. In the growth period of Hela and CNE, they consumed different amino acid. So they need different component proportion for amino acid.

关 键 词：细胞培养 培养基 吸收光谱 芳香族氨基酸 癌细胞生长 

分 类 号：Q813.11[生物学—生物工程]