骨髓动员与骨髓细胞移植对急性心肌梗死治疗作用的比较研究  

Comparative Study of Bone Marrow Cell Transplantation and Mobilization on the Postinfarction Neovascularization and Cardiomyocytes Regeneration in Acute Myocardial Infarction

作　　者：彭建军[1] 路璐[1] 吴旸[2] 胡大一[1] 王长华[1] 朱正炎[1] 李建勇[1] 

机构地区：[1]首都医科大学北京同仁医院心血管疾病诊疗中心,北京100730 [2]北京市海淀医院心内科,北京100080

出　　处：《中国医药导刊》2004年第4期276-278,共3页Chinese Journal of Medicinal Guide

摘　　要：目的:比较骨髓间充质干细胞移植和骨髓动员对急性心肌梗死再通模型心功能作用,及两者心肌及血管再生作用。方法:将小型猪模型分为3组,对照组(5头),骨髓动员组(6头),细胞移植组(8头)。心肌梗死再通模型制作后,骨髓动员组予人重组粒细胞集落刺激因子静脉注射1天,皮下注射连续5天,细胞移植组予骨髓间充质干细胞(4—8)×106经梗死相关冠脉注入。3周后观察以下指标:(1)超声心动图检测左室射血分数。(2)免疫组织化学染色,行不同区域血管计数,并观察增殖期内皮细胞和心肌细胞或再生心肌细胞。结果:(1)骨髓动员组和细胞移植组心功能明显改善。(2)细胞移植组在心肌梗死周围区可见分散分布的BrdU标记并分化为心肌细胞的移植细胞,骨髓动员组中亦观察到处于增殖期的心肌细胞;细胞移植组和骨髓动员组均可见大量新生血管,但二者分布区域不同。结论:骨髓动员及骨髓间充质干细胞移植均可明显改善心肌梗死后心功能,二者可促进心肌细胞再生,二者对不同区域的血管再生作用不同。Objective: To compare the impact of MSCs transplantation and bone marrow mobilization on the heart function, and cardiomyocytes regeneration and angiogenesis in the swine model of post-infarction neovascularization. Methods: Swines were transplanted with MSCs(transplantation group,n =8) or mobilized with G-CSF(mobilization group, n=6) or no therapy method was used(control, n=5) after developing model of post-infarction neovascularization. The measurements includes: evaluating the ventricular function with echocardiography; counting the number of vessels in different area, observing the regeneration of cardiomyocytes. Results:Either mobilization of bone marrow using G-CSF or transplantation of MSCs through coronary can improve the post-infarction heart function, and impel the angiogenesis process through with different impact on each area Conclusions: Mobilization of bone marrow and transplantation of MSCs can both achieve optimal myocytes regeneration and angiogenesis although they focus on different location. So they contribute to the improvement of heart function.

关 键 词：骨髓动员 骨髓细胞移植 急性心肌梗死 治疗 心功能 血管再生 

分 类 号：R542.22[医药卫生—心血管疾病]