白介素-1受体拮抗剂对BXSB狼疮小鼠的治疗作用观察  

Study on the Effect of Continuous Administration of IL-1ra in BXSB Mice

作　　者：杨高云[1] 狄春辉[2] 马大龙[2] 姜学义[2] 李世荫[2] 蒋明[1] 

机构地区：[1]中国医学科学院中国协和医科大学协和医院,北京100730 [2]北京医科大学

出　　处：《中国医学科学院学报》1997年第6期409-413,共5页Acta Academiae Medicinae Sinicae

摘　　要：利用基因工程技术获得高纯度人白介素-1受体拮抗剂（IL－1ra），连续性尾静脉注射4．5月龄雄性BXSB狼疮小鼠，发现其尿蛋白水平和ANA滴度的增加幅度减少，肾脏IgG、C3沉积减轻，血清白细胞介素-6（IL－6）活性和肾脏IL－6蛋白表达减弱。提示IL－1在BXSB狼疮小鼠发病中的病理性作用及IL－1ra对该鼠可能的治疗作用。To explore the consequences of IL-1 blocking in BXSB mice which is an experimental model for human SLE. Methods rh IL^1ra was expressed in E. coli and injected in BXSB mice. 13 of 4. 5 month-age male BXSB mice were divided into two groups, one group was injected rh 1L-1ra 10 times (twice a week) at dose of 400μg per mouse each time,another group was inJected PBS at the same time as control. We monitored serum ANA and proteinuria weekly, and detected IgG, C3 deposition and IL-6 expression in kidneys at 40th day. Results The results showed that the increased level of serum ANA and proteinuria in treatment group were not higher than in control group, the IgG, C, deposition and IL-6 expression in kidneys and IL-6 activity in serum of the treaed group were lower than the control group, whereas no difference of GPT level in the two groups. Conclusion IL-1 might play a pathogenic role in BXSB mice. Blocking or reducing IL-1 secretion would be of beneficial to the treatment of SLE.

关 键 词：白介素—1受体拮抗剂 BXSB小鼠 系统性红斑狼疮 

分 类 号：R593.24[医药卫生—内科学]