雌二醇对大鼠垂体前叶细胞增殖的影响及其机制的初步探讨  

The Effects of E_2 and It's Metabolites on the Proliferation of Rat Anterior Pituitary Cells in vitro

作　　者：狄安稞[1] 许荣焜[1] 吴雪梅[1] 黄曼影[1] 单惠敏[1] 周远征[1] 钱忠明[2] 邓柏澧[2] 彭树熏[3] 何淑芬[3] 

机构地区：[1]中国医学科学院基础医学研究所,中国协和医科大学基础医学院,北京100005 [2]香港理工大学 [3]香港大学医学院

出　　处：《中国医学科学院学报》1997年第6期424-429,共6页Acta Academiae Medicinae Sinicae

基　　金：国家自科学基金!39470276

摘　　要：观察了17-β羟雌二醇（17-β-estradiol，E2）及其代谢产物2-羟雌酮（2-hydroxyestrone，2－OHE1）、2-羟雌二醇（2－hydroxyestradiol，2－OHE2）和多巴胺（dopamine，DA）受体激动剂piribedil对大鼠垂体前叶细胞（aneriorpituitarycells，APC）增殖的影响。结果表明：E2（10－6mol/L）可促进APC生长，A10－5mol/L的piribedil却可抑制APC的增殖活性。提前12h加入E2或2－OHE2、2－OHE2可抑制piribedil的效应，但E2对piribedil效应无明显影响。and Methods The present work is determined to observe the effects of E2 (estradiol ), 2-OHE1 (2-hydroxyestrone ), 2 -OHE2 (2-hydroxyestrodiol ) on the proliferation of rat anterior pituitary cells (APC) in vitro by laser scanning confocal microscopy. Results 10-6mol/L E2 stimulated the growth of APC. After 2 days of incubation with E2, the DNA content of APC increased to 1. 3 times of the control group (P<0. 01 ). 10-6mol/L 2-OHE2 (other than 2-OHE1 ) stimulated proliferative activity of APC and inhibited the inhibitory effect of peribidil (10-5mol/L), a dopamine receptor agonist, on the poliferative activity of rat APC.

关 键 词：17—β羟雌二醇 2-羟雌酮 2-羟雌二醇 PIRIBEDIL 垂体前叶细胞 

分 类 号：Q228[生物学—细胞生物学]