纤维素酶系组成对二次纤维超分子结构形态的影响1  

Effect of Different Cellulase Composition on Secondary Fibers Morphology Ultrastructure

作　　者：景宜[1] 尤纪雪[1] 吴淑芳[1] 徐勇[1] 

机构地区：[1]南京林业大学化学工程学院,江苏南京210037

出　　处：《纤维素科学与技术》2004年第3期1-8,共8页Journal of Cellulose Science and Technology

基　　金：国家自然科学基金资助课题(30271059)

摘　　要：利用两种不同来源的纤维素酶按照不同的滤纸酶活/CMC酶活[Filter Paper Activity/CMCase(F/C)]比值进行混合,酶解二次纤维后,借助电子显微镜、X-射线衍射以及红外光谱等手段,分析酶解对纤维超分子结构的影响。研究结果表明: F/C比值从0增加到2.5,纤维结晶度从65.49%增加到73.40%;F/C增加至3.0,结晶度下降至65.52%。纸张强度随着F/C值增加从21.78 Nm/g下降至16.57 Nm/g。酶解导致纤维细胞致密的次生壁结构疏松多孔,酶解由纤维表面向内层转移,单根纤维强度和成纸强度下降。Two kinds of cellulase were produced from the different origin. They were mixed according to the different rate of filter paper activity to CMCase(F/C). Cellulase could affected the fibers ultrastructure and degraded the fibers. Scanning electron microscope(SEM), X-diffraction and infrared spectra(IR) were the main analysis methods. Results shows crystalline degree of fibers increases from 65.49% to 73.40% and decreases to 65.52% with different F/C. Enzymolysis makes the cell wall loose and porous. Enzyme attacks fibers from the fiber surface to interior. Single fiber and paper strength decrease.

关 键 词：纤维素酶 二次纤维 电子显微 镜 X-射线衍射 红外光谱 酶解 超分子结构 结晶度 生物酶 生物技术 F/C 

分 类 号：TS724[轻工技术与工程—制浆造纸工程] Q814.9[生物学—生物工程]