赤潮藻细胞计数方法比较研究  

Comparative study on cell count method with prorocentrum donghaiense2?

作　　者：侯建军[1] 黄邦钦[1] 戴相辉[1] 

机构地区：[1]海洋环境科学教育部重点实验室/厦门大学环境科学研究中心

出　　处：《中国公共卫生》2004年第8期907-908,共2页Chinese Journal of Public Health

基　　金：国家 973重点基础研究发展项目 (G1 9990 4 370 6);国家自然科学基金项目 (40 3760 4 3;40 0 760 31 )

摘　　要：目的　建立简便、快捷、准确、可行的赤潮藻细胞计数方法。方法　采用显微镜法、可见分光光度法、库尔特法、流式细胞计数显微镜 (FlowCAM)等方法对纯培养的东海原甲藻进行细胞计数。结果　 3种细胞计数法与显微镜计数相比较 ,均有较好的线性相关性。结论　 4种细胞计数方法各有其优缺点 ,其中库尔特计数是相对简便、快捷、准确、可行的细胞计数方法。Objective To found simple,exact and feasible cell count methods.Methods Microscope,visible spectrophotography,FlowCAM (Flow Cytometer and Microscope) and Multisizer TM 3 Coulter counter were used to measure cell amount of prorocentrum donghaiense.Results The insightful comparison on the interrelations of the above 3 mentioned cell count methods was made with the method of microscope,which revealed the sound linear interrelations between them.Conclusion All the 4 mentioned cell count methods had its advantages and disadvantages,among which,the cell count method with Multisizer TM 3 Coulter counter was concise,exact and reliable experimental approach.

关 键 词：东海原甲藻(Prorocenum donghaiense) 赤潮藻细胞细胞计数 库尔特法 

分 类 号：Q949.221[生物学—植物学]