黄檗中生物碱含量的高效液相色谱分析  

DETERMINATION OF ALKALOIDS CONTENT IN PHELLODENDRON AMURENSE RUPR. BY HPLC

作　　者：秦彦杰[1] 张玉红[2] 王洋[1] 阎秀峰[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]东北林业大学森林植物生态学教育部重点实验室,黑龙江哈尔滨150040

出　　处：《林产化学与工业》2004年第B08期115-118,共4页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目 (30 2 710 4 5 );东北林业大学科研基金资助项目 (无项目号 )

摘　　要：建立了一种简便、快速、准确的测定黄檗 (PhellodendronamurenseRupr.)中生物碱含量的高效液相色谱分析方法。色谱条件 :采用日本KYAHIQsilC 18柱 (2 5 0mm× 4 .6mm ,5 μm) ,流动相乙腈 /水溶液 (10 0 0mL溶液中含磷酸二氢钾 3.4 g及十二烷基磺酸钠 1.7g) 1∶1,流速 1.0mL/min ,检测波长 345nm ,进样量 10μL。样品制备以 6 3%的乙醇作溶剂 ,在 4 1℃下超声波提取黄檗茎皮 6 4min。用HPLC分别测定了黄檗根皮、茎皮和叶中小檗碱、掌叶防己碱、药根碱的含量 ,结果表明 :小檗碱和药根碱在根皮中的含量最高 ,茎皮次之 ,叶最低 ,而掌叶防己碱在茎皮中的含量最高 ,根皮次之 ,叶最低。A simple, rapid and sensitive method for the determination of alkaloids content in Phellodendron amurense R. was established. Alkaloids was ultrasonically extracted with 63 % alcohol at 41 ℃ for 64 min, and determined by high performance liquid chromatography. The alkaloids were separated on a KYA HIQ sil C18 column (250 mm×4.6 mm, 5 μm), using acetonitrile-aqueous solution (3.4 g KH_2PO_4 and 1.7 g SDS in 1 000 mL H_2O ) 1∶1 as the mobile phase at flow rate 1.0 mL/min and detected at 345 nm. Contents of berberine, palmatine and jatrorrhizine in the root bark, stem bark and leaves of P. amurense R. were determined. The results showed that alkaloids contents in the root bark, stem bark and leaves were different. Contents of berberine and jatrorrhizine were higher in the root bark than in the stem bark, and they were the lowest in the leaves; while palmatine content was the highest in the stem bark, then in the root bark, and the lowest in the leaves too.

关 键 词：黄檗 生物碱 含量测定 高效液相色谱 超声波提取 

分 类 号：Q949.752.7[生物学—植物学] TQ283[化学工程—有机化工]