紫杉醇诱导Hela细胞凋亡及其与凋亡相关蛋白的关系  

Relationships between induction of apoptosis by Taxol in Hela cells and apoptosis-related proteins

作　　者：胡向阳[1] 孟刚[1] 鲍扬漪[2] 朱晓梅[2] 汪渊[3] 周青[3] 

机构地区：[1]安徽医科大学病理解剖教研室,安徽合肥230032 [2]合肥市第一人民医院肿瘤科,安徽合肥230061 [3]安徽医科大学分子生物学实验室,安徽合肥230032

出　　处：《中国药理学通报》2004年第9期1063-1067,共5页Chinese Pharmacological Bulletin

基　　金：安徽医科大学博士科研基金资助项目;No 5 2 13 77;合肥市科委资助项目 ;No合科 2 0 0 3 -3

摘　　要：目的　探讨Taxol诱导Hela细胞凋亡的特点及细胞凋亡的分子机制。方法　用紫杉醇诱导Hela细胞凋亡 ,采用HE、TUNEL电镜及流式细胞仪分别检测凋亡及观察凋亡细胞的形态变化特点。采用免疫组化S P法及ELISA等分别检测凋亡相关蛋白PCNA及caspase 3的表达和活性的动态变化。结果　紫杉醇可诱导Hela细胞凋亡并可使Hela细胞出现较多的病理性核分裂像以及细胞核的多微核化 ;正常增生的Hela细胞有一定的PCNA表达 ,加入Taxol后 ,与对照组比较 ,其表达明显增强 ;不同浓度的Taxol可使Hela细胞中caspase 3活性的增高 ,并具有时间及剂量的依赖性。结论　Taxol可诱导Hela细胞凋亡 ,Hela细胞凋亡是CaspaseAim To investigate the effects of taxol on apoptosis in Hela cell and the mechanism of apoptosis. The apoptotic cells were detected by TUNEL, HE, eletronic micrpscopy and flow cytometry method. The expressions and activity of apoptosis associated proteins such as PCNA and caspase-3 were examined using S-P and enzyme histochemistric method.The results followed as: HeLa cells exposed to Taxol undergo cell death, presenting morphological and biochemical characteristics typical of apoptosis and the apoptotic cells increased with time and concentration. In contrast to untreated Hela cells, which express low PCNA, Ones treated with Taxol expressed high amounts of PCNA. Conclusion Taxol may induced apoptosis in Hela cell. The apoptosis induced by taxol is related to the increase of PCNA protein and activity of caspase-3.

关 键 词：HELA细胞 紫杉醇 细胞凋亡PCNA CASPASE-3 

分 类 号：R282.71[医药卫生—中药学] R329.25[医药卫生—中医学]