失神经支配大鼠皮肤切割创面愈合过程中修复细胞增生活性的研究  

Study of proliferative activity of repairtive cell on cutaneous excision wounding after denervation rats

作　　者：徐获荣 汤苏阳[2] 刘春杰 

机构地区：[1]锦州市法医鉴定所,辽宁省锦州市121000 [2]北京武警总医院烧伤整形外科

出　　处：《中国美容医学》2004年第5期529-530,共2页Chinese Journal of Aesthetic Medicine

摘　　要：目的:研究大鼠失神经支配皮肤切割伤愈合过程中修复细胞增生活性的变化。方法:40只SD大鼠随机分成T、C两组(每组20只),在其背部形成全层皮肤切割伤缺损创面,其中T组为手术损伤鼠T11～L12脊神经皮肤切割伤组,C组为正常有神经支配的皮肤切割伤组,在伤后1、2、3、4周,观察创面愈合,A、B两组同时取材,采用免疫组化和银染法,检测创面修复细胞PCNA的表达和AgNORs颗粒数的变化。结果:T组较C组创面愈合明显延迟,同时修复细胞PCNA表达明显减弱,细胞核内AgNORs颗粒数明显减少,第3周两组的创面PCNA标记指数和AgNORs计数分别为2.11%/18.1%(P=0.001),1.1/3.1个/细胞(P<0.05)。结论:大鼠失神经支配皮肤切割伤愈合较正常创面愈合明显延迟,修复细胞增殖活性明显降低。Objective To study the proliferative activity of the repair cell on cutaneous wound healing after denervation rats. Methods 40 Sprague-Dawley rats were randomly and equally divide into T group,C group(20 in each group), that T,C group were created the dorsal cutaneous excision wounding in which the T group were denervated the dorsal wounds of rats, the C group were normal as the controls ,At1,2,3,4,weeks after wounding. The change of cutaneous wound healing was investigated, the expression of proliferation cell nuclear antigen (PCNA )was detected via immmunhischemical method and the granular numbers of argyophilic protein of the nucleoiar organizer regions (AgNORs) by silver staining. Results The cutaneous wound healing was delayed meanwhile The labelling index of PCNA and the granular numbers of AgNORs were reduced by denervation in both early and late stages of healing in T group than that in C group significantly such as the labelling index of PCNA of T group and C group after cutaneous wound 3 weeks was 2.11%/18.1%(P=0.001) the granular numbers of AgNORs was 1.1/3.1/cell (P<0.05). Conclusion The cutaneous excision wound healing was delayed and proliferative activity was decreased by denervation significantly.

关 键 词：皮肤切割伤 失神经支配 修复细胞 增生活性 

分 类 号：R641[医药卫生—外科学]