卡介菌多糖核酸对正常小鼠T淋巴细胞亚群的影响  

EFFECTS OF BCG-POLYSACCHARIDES NUCLEIC ACID ON T-LYMPHOCYTE SUBSETS IN NORMAL MICE

作　　者：李永喜[1] 陈兴平[2] 邓云华[2] 陈映玲[2] 周礼义[2] 

机构地区：[1]青岛市市立医院皮肤科,山东青岛266011 [2]华中科技大学同济医学院附属同济医院皮肤科

出　　处：《青岛大学医学院学报》2004年第4期328-330,共3页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：①目的　探讨卡介菌多糖核酸对正常小鼠T淋巴细胞亚群的影响及其免疫调节的机制。②方法用流式细胞仪测量小鼠胸腺、脾脏、外周血单个核细胞中T细胞亚群CD+ 4 、CD+ 8细胞变化并计算CD+ 4 /CD+ 8的比值。③结果　与正常对照组比较 ,卡介菌多糖核酸可提高正常小鼠上述 3种不同组织中CD+ 4 、CD+ 8细胞的比例 ,并在第 35天有明显差异 (F =5 .6 3～ 1 1 .2 1 ,q =5 .32～ 9.1 3,P <0 .0 5 ) ,而对CD+ 4 /CD+ 8比值无明显影响。④结论卡介菌多糖核酸可以促进T细胞的分化与成熟 ,并可能通过调节CD+ 4 、CD+ 8细胞两亚群之间的平衡来发挥免疫调节作用。Objective To investigate the effects of BCG-polysaccharides nucleic acid (BCG-PSN) on T-lymphocyte subsets of normal mice and its role in immunoregulation. Methods The changes of CD + 4 and CD + 8 lymphocytes were measured with flow cytometry in the thymus, spleen and peripheral blood of the mice, and the CD + 4 / CD + 8 ratio was calculated. Results Compared with the control group, BCG-PSN could increase CD + 4 and CD + 8 lymphocytes in the above-mentioned tissues, the difference became significant on the 35th day ( F=5.63-11.21, q=5.32-9.13, P <0.05), but it had no effect on the CD + 4 / CD + 8 ratio. Conclusion BCG-PSN can accelerate the differentiation and maturity of T-lymphocytes and may exerts its immunoregulative role by regulating the balance between CD + 4 and CD + 8.

关 键 词：卡介菌多糖核酸 T淋巴细胞 免疫测定 小鼠 

分 类 号：R967[医药卫生—药理学]