DNA与生物碱类化合物非共价作用的负离子电喷雾质谱研究(Ⅰ)  

Non-covalent Interaction Between Oligodeoxynucleotide and Alkaloid Compounds via Negative Ion Electrospray Mass Spectrometry(Ⅰ)

作　　者：宋凤瑞[1] 刘宁[1] 闫存玉[1] 刘志强[1] 刘淑莹[1] 

机构地区：[1]中国科学院长春应用化学研究所新药研究实验室,长春130022

出　　处：《高等学校化学学报》2004年第10期1830-1832,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 173 0 5 7);吉林省科技厅科技专项基金 (批准号 :2 0 0 3 0 5 0 2 )资助

摘　　要：Oligodeoxynucleotide from SARS virus was selected and synthesized. Its complexes with alkaloid compounds were investigated by electrospray mass spectrometry. We found that three alkaloid compounds could interact with the DNA target molecule in five alkaloids analyzed. With increasing molar ratio of the three alkaloid compounds, the complexes between alkaloids and DNA with different stoichiometric ratios were found in their MS spectra. The order of the alkalinity of their gas phase corresponds to that of liquid phase, that is: Palmatine>Jatrorrhizine>Berberin. The relative abundance of these complexes in mass spectra can assess the relative affinities of above three alkaloid compounds with the DNA target molecule. In this paper, the binding sites between jatrorrhizine and DNA was deduced from the interaction experiments of jatrorrhizine with three nucleosides.Oligodeoxynucleotide from SARS virus was selected and synthesized. Its complexes with alkaloid compounds were investigated by electrospray mass spectrometry. We found that three alkaloid compounds could interact with the DNA target molecule in five alkaloids analyzed. With increasing molar ratio of the three alkaloid compounds, the complexes between alkaloids and DNA with different stoichiometric ratios were found in their MS spectra. The order of the alkalinity of their gas phase corresponds to that of liquid phase, that is: Palmatine>Jatrorrhizine>Berberin. The relative abundance of these complexes in mass spectra can assess the relative affinities of above three alkaloid compounds with the DNA target molecule. In this paper, the binding sites between jatrorrhizine and DNA was deduced from the interaction experiments of jatrorrhizine with three nucleosides.

关 键 词：DNA 生物碱类化合物 非共价复合作用 负离子电喷雾质谱 抗病毒药物 药物筛选 

分 类 号：Q523[生物学—生物化学] R978.7[医药卫生—药品]