反应时间、温度和溶剂对缬氨酸自组装成聚合肽反应的影响  

Influence of Reaction Time, Temperature and Solvent on the Self-assembly into Oligo-peptides for Valine

作　　者：李红[1] 赵文杰[1] 曹书霞[2] 卢奎[1] 赵玉芬[2] 

机构地区：[1]河南工业大学化学化工系,郑州450052 [2]郑州大学化学系化学生物学重点实验室,郑州450052

出　　处：《高等学校化学学报》2004年第10期1866-1868,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 2 72 0 5 5 );河南省杰出青年科学基金 (批准号 :0 3 12 0 0 0 90 0 )资助

摘　　要：Owing to having very strong bioactivity, oligo-peptides can be used as the medication or the pre-medication and become the studying focus now. Existing methods for synthesizing oligo-peptides included liquid phase peptide synthesis, solid phase peptide synthesis and enzymatic peptide synthesis. In this paper, a new method for synthesizing oligo-peptides by phosphorus oxychloride activation was reported. With the assistance of phosphorus oxychloride, the phosphorylation of L-valine could take place and then was assembled into oligo-peptides, which were analyzed by electrospray ionization mass spectrometry(ESI-MS). On quenching with methanol, the reaction mixtures, which were produced under different reaction conditions, yielded the corresponding peptide methyl esters. Moreover, we found that, as the reaction conditions such as reaction time, solvent and temperature were changed, the length of peptides changed. And the length of peptides could be controlled by changing the reaction conditions.Owing to having very strong bioactivity, oligo-peptides can be used as the medication or the pre-medication and become the studying focus now. Existing methods for synthesizing oligo-peptides included liquid phase peptide synthesis, solid phase peptide synthesis and enzymatic peptide synthesis. In this paper, a new method for synthesizing oligo-peptides by phosphorus oxychloride activation was reported. With the assistance of phosphorus oxychloride, the phosphorylation of L-valine could take place and then was assembled into oligo-peptides, which were analyzed by electrospray ionization mass spectrometry(ESI-MS). On quenching with methanol, the reaction mixtures, which were produced under different reaction conditions, yielded the corresponding peptide methyl esters. Moreover, we found that, as the reaction conditions such as reaction time, solvent and temperature were changed, the length of peptides changed. And the length of peptides could be controlled by changing the reaction conditions.

关 键 词：反应时间 温度 溶剂 缬氨酸 自组装反应 聚合肽 链锁反应 正离子电喷雾质谱 

分 类 号：TQ464.7[化学工程—制药化工]