MALDI-TOF质谱和电子光谱技术研究人血清铁蛋白释放铁的动力学  

Kinetics Study of Iron Release of Human Serum Ferritin with MALDI-TOF Mass Spectrometry and Electronic Spectrum Technology

作　　者：颜利[1] 黄河清[1] 金宏伟[1] 陈平[1] 杨天赐[1] 王群力[1] 

机构地区：[1]厦门大学生命科学学院

出　　处：《高等学校化学学报》2004年第10期1889-1892,共4页Chemical Journal of Chinese Universities

基　　金：厦门市自然科学基金 (批准号 :3 5 0 2 Z2 0 0 12 63 )资助

摘　　要：采用 MALDI-TOF质谱技术研究血清混合蛋白质的组成 ,用电子光谱技术分析人血清铁蛋白 ( Serumferritin,SF)释放铁的动力学过程和规律 .结果表明 ,在人血清中 ,以 Na2 S2 O4 为电子供体时 ,SF释放铁的速率极快 ,无法采用常规分光光度法进行检测 ;以抗坏血酸为电子供体时 ,SF以一级反应动力学方式释放铁 ,认为人血清中其它蛋白质协助或参与调控铁蛋白进行快速释放铁反应 。MALDI-TOF mass spectrometry was used to understand the compound of proteins in human serum and electronic spectrum was used to study the kinetics of iron release of the human serum ferritin. The result shows that if Na 2S 2O 4 was taken as the reducer, it is impossible to examine the iron release in serum with normal spectrum. When Vc was used as the reducer, the kinetic reaction of the iron release from serum ferritin was the first-order. It might be due to the abundance and influence of other proteins in human serum in the course of iron release, and that could fit the need of iron in the body.

关 键 词：人血清铁蛋白 释放铁 动力学 MALDI-TOF质谱技术 电子光谱技术 

分 类 号：Q513[生物学—生物化学]