杆状病毒SpltMNPV病毒粒子中存在泛素复合物  

Ubiquitin-conjugates were Found in Spodoptera litura Nucleopolyhedrovirus Particles

作　　者：李朝飞[1] 尹崇[1] 何敏儿[1] 庞义[1] 

机构地区：[1]中山大学生物防治国家重点实验室∥昆虫研究所,广东广州510275

出　　处：《中山大学学报（自然科学版）》2004年第5期66-68,共3页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家重点基础规划(973)资助项目(G2000016209);中山大学青年教师启动基金资助项目

摘　　要：通过间接免疫荧光分析,发现在斜纹夜蛾核多角体病毒(Spodopteralituranucleopolyhedrovirus,SpltMNPV)感染的Sl＿zsu＿1细胞的细胞质中存在大量的泛素或泛素复合物。Western印迹分析表明,在SpltMNPV多角体源病毒粒子与芽生型病毒粒子中均存在相对分子质量为30×103～65×103的泛素复合物。推测SpltMNPV在出芽或组装的过程中由感染细胞的胞质中获得泛素复合物。Insect viruses are the only viruses known to encode ubiquitin. Indirect immunofluorescence analysis indicated that ubiquitin or ubiquitin-conjugates were distributed in the cytoplasm of Spodoptera litura nucleopolyhedrovirus (SpltMNPV)-infected Sl-zsu-1 cells. Western blot analysis revealed that 3 000～6 5000 ubiquitin-conjugates were located in SpltMNPV ODV and BV, respectively. It was proposed that the ubiquitin-conjugates in SpltMNPV structure were obtained in the process of virus budding or assembly.

关 键 词：斜纹夜蛾核多角体病毒 SPLTMNPV 泛素 

分 类 号：Q965[生物学—昆虫学]