粉红聚端孢菌TrEn39 cDNA基因的原核表达  

Prokaryotic Expression of the TrEn39 cDNA of Trichothecium roseum in Escherichia coli

作　　者：魏毅 刘开启 张传云 张世宏[3] 李多川[4] 潘洪玉[5] 

机构地区：[1]山东省棉花研究中心,济南250100 [2]广州仲铠农业技术学院植物保护系,广州510000 [3]中国农业大学植物病理系,北京100094 [4]山东农业大学植物病理系,泰安271018 [5]解放军军需大学农副业生产系,长春130062

出　　处：《吉林农业大学学报》2004年第4期399-401,410,共4页Journal of Jilin Agricultural University

基　　金：教育部博士点基金资助项目

摘　　要：作者曾经克隆了粉红聚端孢S24的TrEn39基因(AY550119),根据基因获得了其可能的cDNA序列。在此基础上,构建了TrEn39cDNA的原核表达载体,并研究了其在大肠杆菌中表达产物的酶活性,从而确认所克隆的基因为粉红聚端孢S24几丁质酶(TrEn39)的cDNA。The authors cloned the gene TrEn39 DNA (AY550119) of Trichothecium roseum S24 and obtained the probable sequence of cDNA according to the gene. In order to further understand the characteristics and functions of this gene, we then constructed the prokaryotic expression vector of the cDNA and tried to make it express in Escherichia coli. And it was confirmed that the cDNA we cloned was chitinase TrEn39 cDNA of Trichothecium roseum S24.

关 键 词：粉红聚端孢S24 TrEn39 CDNA 原核表达 

分 类 号：Q949.32[生物学—植物学]