8种基因型的高羊茅的组织培养与植株再生  

Tissue culture and plant regeneration of eight genotypes of tall fescue

作　　者：支大英[1] 韩晓光[2] 赵军胜[1] 薛哲勇[1] 夏光敏[1] 

机构地区：[1]山东大学生命科学学院,山东济南250100 [2]河北廊坊师范学院生物系,河北廊坊065000

出　　处：《山东大学学报（理学版）》2004年第4期109-114,共6页Journal of Shandong University(Natural Science)

基　　金：国家转基因专项资助项目 (J990 3 7)

摘　　要：以 8个品种的高羊茅下胚轴为外植体 ,分别在诱导培养基D1、D3、D5和D9上培养 ,除D1培养基上的愈伤组织的出愈率为 5 1.3%外 ,其他诱导培养基上的出愈率均为 10 0 % .其中在D5培养基上继代 3个月左右 ,获得了胚性愈伤组织 ,此胚性愈伤组织在F1分化培养基上的分化率达到 70 %～ 80 % .在较长时间的继代培养中 ,D3培养基可以保持愈伤组织的高分化率 .Hypocotyls of eight breeds of tall fescue were cultured on the callus induce medium D1, D3, D5 and D9 respectively. Except the rate of callus grown on medium D1 was 51.7%, all the callus were growing from the explants with the rates at 100% on the other mediums. The embryonic callus were induced from callus-induction medium D5 cultured after subcultured for 3 months. The rate of shoot regeneration from embryogenic callus on the differentiation medium F1 was 70%～80%. In long term subculture, the embryogenic callus have high regenerated rates on medium D3.

关 键 词：高羊茅 胚性愈伤组织 组织培养 植株再生 

分 类 号：Q943.1[生物学—植物学]