双波长法用于磺酸型树脂吸附BSA和trypsin的研究  

Study on the dual-wavelength spectrophotometric determination of adsorption of BSA and trypsin on sulfuric acidic cation exchange resins

作　　者：魏荣卿[1] 邵勇军[1] 刘晓宁[1] 张婷婷[1] 姚忠[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《生物加工过程》2004年第3期56-59,共4页Chinese Journal of Bioprocess Engineering

基　　金：江苏省科技攻关项目 (BE2 0 0 10 40 )

摘　　要：考察了H+ 和Na+ 变化对紫外吸收单波长法、双波长法和考马斯亮蓝法的影响。结果表明双波长法比考马斯亮蓝法和单波长法稳定性要好。选择双波长法用于磺酸型离子交换树脂吸附胰蛋白酶 (trypsin)和牛血清白蛋白(BSA)的检测 ,结果表明 :交换容量为 4 88mmol/g的树脂对BSA和trypsin的吸附速率和饱和吸附量均高于交换容量为 3 17mmol/g的树脂。The influence of H + and Na + on the mono - wavelength spectrophotometry,dual-wavelength spectrophotometry and Bradford me thod was investigated The results showed that the dual-wavelength method was more stable than other two methods The dual-wavelength method was applied i n the determination of adsorpt io n of BSA and trypsin on sulfuric acidic cation exchange resins,the resin with e xchange capacity of 488 mmol/g has higher maximum adsorptive capacity and adso rptive velocity of BSA and trypsin than the resin of 488 mmol/g

关 键 词：双波长法 磺酸型离子交换树脂 牛血清白蛋白 胰蛋白酶 

分 类 号：O65[理学—分析化学]