辅酶I在Co/CFUE上的电化学行为及应用  

Study on the electrochemical behavior of NAD^+ at Co ion implanted modified carbon fiber ultramicroelectrodes

作　　者：王静[1] 胡劲波[1] 李启隆[1] 

机构地区：[1]北京师范大学化学系,北京100875

出　　处：《分析试验室》2004年第9期1-4,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金资助课题(20275007)

摘　　要：辅酶I(NAD+)在0.005moL/LTris 0.01moL/LNaCl溶液(pH7.0)中,于钴离子注入修饰碳纤维电极上出现一个S形的还原波,半波电位为-1.45V(vs.SCE)。峰电流与NAD+的浓度在2.38×10-5～4.76×10-4mol/L(r=0 9992)和4.76×10-4～1.78×10-3mol/L(r=0.9982)之间成线性关系,检出限为1.19×10-5mol/L,回收率在96.7%～103.4%之间。用线性扫描、循环伏安法研究了钴离子注入修饰碳纤维电极上NAD+的电化学行为。电极反应机理为:NAD++e NAD·;NAD·+e+H+ NADH;2NAD·→NAD2。另外,钴离子注入修饰碳纤维电极对NAD+具有电催化作用。With Co ion implanted modified carbon fiber ultramicroelectrode (Co/CFUE) as the working electrode, in 0.005 moL/L Tris-0.01 moL/L NaCl (pH 7.0) buffer solution, a S-shape reduction wave of NAD^+ was obtained by linear sweep voltammetry. The half wave potential was at -1.45 V(vs. SCE). The reduction current was proportional to the concerntration of NAD^+ over the range of 2.38×10^(-5)～4.76×10^(-4) mol/L(r=0.9992) and 4.76×10^(-4)～1.78×10^(-3) mol/L(r=0.9982), with the detection limit of 1.19×10^(-5) mol/L and recovery of 96.7%～103.4%. The electrochemical behavior of NAD^+ was studied by linear sweep voltammetry and cyclic voltammetry.

关 键 词：辅酶Ⅰ 电化学行为 钴离子注入 修饰碳纤维电极 催化 

分 类 号：O657.3[理学—分析化学]