草酸氧化酶基因转化烟草的研究  

Study on transformation of Nicotiana tabacum with oxalate oxidase gene

作　　者：景岚[1] 康振生[1] 孙燕飞[1] 张岗[1] 

机构地区：[1]西北农林科技大学植物保护学院陕西杨陵

出　　处：《西北植物学报》2004年第10期1845-1849,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：陕西省农业分子生物学重点实验室基金资助项目;陕西省自然科学基金项目

摘　　要：为研究草酸氧化酶基因(OxO)对植物抗病的作用,将含有CaMV35s启动子的植物表达载体以根癌农杆菌(Agrobacteriumtumefaciens)介导的叶盘方法,转化了烟草97131。具有卡那霉素抗性的再生植株经PCR检测,得到了与阳性对照一致的470bp的片段,进一步对PCR产物测序表明OxO基因已整合进烟草基因组中。在对草酸的耐受性实验中,转基因烟草对草酸的抗性明显高于未转化烟草。In order to study the possibility of utilizing oxalate oxidase gene ( OxO ) to increase plant's resistance, the leaf discs from Nicotiana tabacum line 97131 were transformed by using Agrobacterium tumefaciens harbouring the plasmid containing OxO gene and CaMV 35s promoter. PCR identification with the kanamycin resistant shoots showed the most of the green shoots amplified gave the expected fragment of 470bp as the positive control. Moreover, the PCR products were sequenced. The sequence comparison result showed OxO gene had been integrated into Nicotiana tabacum genome. Treated with different concentrations of oxalic acid, transgenic plants showed a stronger tolerance against oxalic acid than wild type.

关 键 词：草酸氧化酶基因(OxO) 根癌农杆菌介导转化 转基因烟草 耐受性 

分 类 号：Q943.2[生物学—植物学] S572[农业科学—烟草工业]