采用RAPD分析臭鼻克雷伯菌临床菌株的基因型  

Genotyping of Klebsiella pneumoniae subsp.ozaenae Isolates by RAPD

作　　者：马佳毓[1] 张文莉[1] 付英梅[1] 叶杨芹[1] 

机构地区：[1]哈尔滨医科大学微生物教研室,黑龙江哈尔滨150086

出　　处：《微生物学杂志》2004年第5期106-107,共2页Journal of Microbiology

基　　金：国家自然科学基金资助项目 ( 3 0 170 816)

摘　　要：臭鼻克雷伯菌是重要的条件致病菌 ,常引起老人及婴幼儿重症护理病房的交叉感染及爆发流行 ,对其进行流行病学监测有重要的医学意义。用随机引物聚合酶链式反应 (RAPD)对 37株臭鼻克雷伯菌临床菌株基因组DNA进行分型 ,分析扩增产物的指纹图谱。实验结果表明 ,37株臭鼻克雷伯菌RAPD后可出现不同的指纹图谱 ,根据特异泳带C ,J ,F ,Q的有无 ,可将其分 7型 (I～VII)。RAPD可用于检测臭鼻克雷伯菌基因组DNA的异质性 ,是医院内感染分子流行病学研究的好方法。Klebsiella penumoniae subsp.ozaenae is an important opportunistic pathogen. It can frequently cause cross infection and outbreak in the aged and newborn individuals in ICUs. So epidemiological monitor of the microbe is medically significant. We used RAPD to genotype the genomic DNAs from 37 isolates of K.pneumoniae subsp.ozaenae and the fingerprints of the amplification products were analyzed. The results revealed that the 37 isolates of K.pneumoniae subsp.ozaenae offered the different fingerprints after RAPD and could be divided into 7 gonotypes (I～VII) based on the presence of specific fragments C, J, F and Q. In conclusion, RAPD can be used to determine the genomic DNA polymorphism of K.penumoniae subsp.ozaenae, and is a fine molecular epidemiological method in investigation of nosocomial infection.;

关 键 词：臭鼻克雷伯菌 随机引物聚合酶链式反应 

分 类 号：R378.996[医药卫生—病原生物学]