中耳胆脂瘤基质金属蛋白酶2,9的活性检测及临床意义  

Detection of the activity of MMP2 and MMP9 in middle ear cholesteatoma

作　　者：王辉兵[1] 徐志文[1] 唐安洲[1] 苏纪平[1] 

机构地区：[1]广西医科大学第一附属医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉科杂志》2004年第10期620-622,共3页Journal of Clinical Otorhinolaryngology

基　　金：广西自然科学基金资助项目 (桂科自 0 135 0 10 )

摘　　要：目的 :探讨中耳胆脂瘤基质金属蛋白酶 (matrixmetalloproteinases,MMP) 2 ,9的活性水平及临床意义。方法 :应用明胶酶谱法测定 4 1例中耳胆脂瘤和 2 0例外耳道皮肤中MMP2 ,9的活性。结果 :胆脂瘤组织中MMP2和MMP9的平均活性为 (0 .95 4± 0 .4 11)和 (2 .6 76± 0 .734)灰度面积·mg-1·ml-1,而外耳道皮肤的平均活性为 (0 .35 5± 0 .16 0 )和 (1.16 6± 0 .4 4 3)灰度面积·mg-1·ml-1,胆脂瘤组织MMP2和MMP9的活性均较外耳道皮肤高 ,P <0 .0 1,其中MMP9的活性较MMP2高 ,P <0 .0 1,两者呈正相关 ,P <0 .0 1。广泛型胆脂瘤组织中MMP2和MMP9的平均活性为 (1.0 6 2± 0 .4 0 1)和 (2 .94 6± 0 .134)灰度面积·mg-1·ml-1,而局限型胆脂瘤的平均活性为 (0 .70 1± 0 .318)和 (2 .193± 0 .6 0 1)灰度面积·mg-1·ml-1,广泛型胆脂瘤MMP2和MMP9活性较局限型胆脂瘤高 ,P <0 .0 1。复发性与初发性胆脂瘤中MMP2和MMP9活性比较差异均无统计学意义 ,P >0 .0 5。结论 :中耳胆脂瘤中MMP2 ,9活性高于外耳道皮肤 ,且广泛型胆脂瘤高于局限型胆脂瘤 ,提示MMP2 。Objective:To study the relationship between the activity of MMP2, and bone resorption in middle ear cholesteatoma.Method:Specimens from 41 cases of middle ear cholesteatoma and 20 cases of external auditory canal skin were analysed for molecular corresponding to MMP2 and MMP9 by using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis(SDS-PAGE) Zymography.Result:The active levels of MMP2 and MMP9 in the cholesteatoma,which were ( 0.954± 0.411) and ( 2.676± 0.734) respectively,were obviously higher than that in the external auditory canal skins,which were ( 0.355± 0.160) and ( 1.166± 0.443) gray area·mg -1·ml -1 respectively,and showed close relationship (P< 0.01). The active levels of MMP2 and MMP9 in extensive cholesteatoma,which were ( 1.062± 0.401) and ( 2.946± 0.134) respectively, were significantly increased in comparison with in localized cholesteatoma which were ( 0.701± 0.318) and ( 2.193± 0.601) gray area·mg -1·ml -1 respectively.No significant difference was observed between recurrent cases and first cases(P> 0.05).Conclusion:The increase of activity of MMP2 and MMP9 in cholesteatoma indicated that MMP2 and MMP9 may play a vital role in the bone destruction associated with cholesteatoma.

关 键 词：胆脂瘤 中耳 基质金属蛋白酶 

分 类 号：R764.21[医药卫生—耳鼻咽喉科]