BTB/POZ结构域蛋白GRP对热休克中hsp900α基因表达的增强作用  

Enhanced Action of a BTB/POZ Domain Protein on the Expression of hsp90α Gene in Heat Shock

作　　者：陈永军[1] 莫志成[1] 张旌[1] 张业[1] 吴宁华[1] 沈珝琲[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所分子生物学国家重点实验室,北京100005

出　　处：《中国医学科学院学报》2004年第4期392-395,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(39930050)资助~~

摘　　要：目的 探讨人BTB/POZ结构域GAGA元件结合相关蛋白(GRP)在hsp90α基因表达中的作用。方法 用正义或反义GRP真核表达质粒或空载体,分别与装有hsp90α基因启动子(-1756-+37)的pREP4 episomalvector质粒和对照质粒pMCAT共转染Jurkat细胞,提取总RNA,用竞争性半定量RT-PCR测定hsp90α-氯霉素乙酰基转移酶(CAT)报告基因相对启动子活性。结果 热休克时,GRP明显促进pREP4 episomal vector质粒上hsp90α-CAT报告基因启动子活性。结论 GRP可能通过参与染色质动态调整促进pREP4 episomal vector质粒上hsp900α CAT的表达。Objective To study the role of a BTB/POZ domain protein in the expression of hsp90α gene. Methods The eukaryotic expression plasmids of sense- and antisense-GAGA related protein (GRP) or empty vector were transfected into Jurkat cells with pREP4 episomal vector plasmids carrying the hsp90α promoter sequence from -1756 to +37 and control plasmids pMCAT. Total RNA was extracted. The relative promoter activity of hsp90α-CAT reporter gene was determined by competitive RT-PCR assay. Results GRP markly increased the relative promoter activity of hsp90α-CAT reporter gene during heat shock. Conclusion GRP may promote the expression of hsp90α gene by participating in chromatin remolding.

关 键 词：GAGA元件结合相关蛋白 pREP4 episomal VECTOR hsp90a 

分 类 号：Q71[生物学—分子生物学]