荧光桃红-小檗碱缔合微粒体系的光谱特性研究及分析应用  

Study on Spectral Properties of the Associated Particle of Berberine-PHLO and Its Analytical Application

作　　者：康彩艳[1] 蒋治良[1] 莫蔚明[2] 

机构地区：[1]广西师范大学资源与环境学系,广西桂林541004 [2]广西师范大学化学系,广西桂林541004

出　　处：《光谱学与光谱分析》2004年第9期1089-1092,共4页Spectroscopy and Spectral Analysis

基　　金：广西自然科学基金资助

摘　　要：在 pH 6 6的磷酸盐缓冲溶液中 ,荧光桃红在 5 2 0nm有一个吸收峰 ,在 5 6 0nm处有一个荧光峰。当有小檗碱存在时 ,荧光桃红与小檗碱可形成稳定的紫红色缔合微粒。其最大吸收波长在 5 6 0nm ,小檗碱浓度 (c)在 6 6 5× 10 -7～ 7 71× 10 -5mol·L-1范围内符合比尔定律 ,回归方程为A =1 0 5 1× 10 4c+0 0 0 86 ,相关系数为 0 996 9,摩尔吸光系数为 2 2 1× 10 4L·mol-1·cm-1。荧光桃红 小檗碱体系的光谱特性研究表明 ,小檗碱与荧光桃红主要通过静电引力形成疏水性的缔合微粒 ,在 385 ,4 70 ,5 86nm产生 3个共振散射峰 ,5 6 0nm荧光峰的降低是由于复合微粒形成所致。In neutral medium, berberine forms a red-violet association microparticle with PHLO which has an maxmimum absorption at 560 nm, Beer's law is obeyed for berberine in the concentration range of 6.65 x 10(-7)-7.71 x 10(-5) mol (.) L-1. Spectral properties of the berberine-PHLO system were studied. In neutral medium, there are a maximum absorption peak at 520 nm and a fluorescence peak at 560 nm for PHLO. Berberine and PHLO combine into a hydrophobic berberine-PHLO association molecule by static gravitation. With stronger Van der Waals and hydrophobic forces among berberine-PHLO molecules, it forms association particle in red-violet which exhibits three resonance scattering peaks at 385, 470 and 586 nm, respectively. The fluorescence quenching at 560 nm is because the PHLO molecule in the body of the particle may not be excited by incident photons, so it does not emit fluorescence.

关 键 词：荧光桃红 小檗碱 缔合微粒 静电引力 共振散射光谱 分光光度法 荧光光谱 

分 类 号：Q58[生物学—生物化学]