肝肾宝对5/6肾切除大鼠肾组织细胞外基质影响的实验研究  

Experimental Study on the Influence of Ganshenbao on the Extracellular Matrix of Renal Tissue in the Rat with 5/6 Nephrectomy

作　　者：赵宗江[1] 牛建昭[1] 张新雪[1] 杨美娟[1] 赵丽云[1] 魏民[1] 贲长恩[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《北京中医药大学学报》2004年第5期26-29,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家中医药管理局中医药科研基金资助项目 (No 2 0 0 0J P 3 1);国家自然科学基金重点资助项目 (No 3 0 13 0 2 2 0 )

摘　　要：目的　研究肝肾宝对 5 / 6肾切除大鼠肾组织细胞外基质 (ECM)如纤维粘连蛋白 (FN)、层粘连蛋白 (LN)、Ⅳ型胶原 (ColⅣ )蛋白及其基因表达的影响。方法　采用免疫组化、原位杂交方法检测肾组织FN、LN、ColⅣ及相应mRNA表达 ,使用TN - 85 0 2多功能图像分析系统对结果进行处理 ,采用SPSS统计软件统计。结果　显示正常对照组、假手术组 ,均未见FN、LN、ColⅣ蛋白及其mRNA阳性反应物。 5 / 6NT组 ,肾小球细胞细胞浆、肾小管细胞浆见FN、LN、ColⅣ蛋白及其mRNA强阳性黄色反应物。缬沙坦组FN、LN、ColⅣ蛋白及其mRNA阳性反应物减少 ,与 5 / 6NT组比较 ,P <0 0 5 ;肝肾宝组FN、LN、ColⅣ蛋白及其mRNA阳性反应物明显减少 ,与 5 / 6NT组比较 ,P <0 0 1。结论　肝肾宝下调肾组织FN、LN、ColⅣ蛋白及其基因表达 。Objective To study the influence of Ganshenbo on the components of extracellular matrix of renal tissue, such as fibronetin (FN), laminin (LN), and collangen Ⅳ (Col Ⅳ), and on the gene expressions of the extracellular matrix, in the rat with 5/6 nephrectomy (5/6 NT). Methods Immunohistochemical techniques and hybridization in situ were used to detect FN, LN and Col Ⅳ and their mRNA expressions, TN-8502 multi-functional graph analysis system was used to process the experimental data, and SPSS statistical software was used to conduct the statistical processing. Results It was showed that there were no FN, LN and Col Ⅳ and their positive reactants of mRNA found in the rats in both the normal control group and sham-operation group; FN, LN and Col Ⅳ and their intensely-positive yellow reactants of mRNA were found in glomerular cytoplasm and tubular cytoplasm in the rats in the 5/6 NT group; FN, LN and Col Ⅳ and their positive reactants of mRNA were found decreased in the rats in the valsartan group, as compared with those in the 5/6 NT group (P<0.05); and FN, LN and Col Ⅳ and their positive reactants of mRNA were found markedly decreased in the Ganshenbao group, as compared with those in the 5/6 NT group (P<0.01).Conclusion One of the mechanisms of preventing and treating renal fibrosis of Ganshenbao is down-regulating FN, LN and Col Ⅳ and their gene expressions in the renal tissue.

关 键 词：肝肾宝 5/6肾切除 肾纤维化 细胞外基质 

分 类 号：R285.5[医药卫生—中药学]