大鼠骨髓基质细胞的体外分离、培养和鉴定的实验研究  

In vitroisolation, cultivation, and identific ation of bone marrow stromal cells of rats

作　　者：何志义[1] 原丽英[1] 陈晏[1] 赵晶[1] 邹巧治[1] 高卓[1] 欧阳嶷[1] 

机构地区：[1]中国医科大学附属第一医院神经内科,辽宁沈阳110001

出　　处：《中国医科大学学报》2004年第5期395-396,共2页Journal of China Medical University

基　　金：辽宁省自然科学基金资助项目 ( 2 0 0 3 2 0 5 4)

摘　　要：目的 :观察大鼠骨髓基质细胞在体外培养的条件及生物学特性并加以鉴定。方法 :取Wistar大鼠骨髓 ,分离培养骨髓基质细胞 (BMSC) ,相差显微镜下观察其形态 ,传代后观察其生长特性 ,用免疫组化方法加以鉴定。结果 :原代培养 2 0d后可分离得到BMSC ,在 5代以前生长形态相对稳定 ,典型的BMSC可分为两个类型。结论 :BM SC具有贴壁生长特性 ,较易对其进行分离扩增 ,增殖速度快 ,可用于多种疾病的细胞治疗。Objective: To observe in vitro culturing con dition and biological characteristics of bone marrow stromal cells(BMSC) of Wist ar rats and identify the cells. Methods: We separated bone mar row of the Wistar rats and cultured the BMSCs. The morphology of the cells was s tudied with a phase contrast microscope and the living characteristics after su bculture of the cells were observed. We identified the cells by using immunohist ochemical assessment. Results: BMSC could be separated 20 days after primary culture. The living characteristics of the cultured cells were qu ite stable before the 5th generation. Typical BMSC could be divided into 2 types . Conclusion: BMSC, which can adhere the wall, can be easily s eparated and quickly proliferated by culture, and can be used in cell therapy in many kinds of diseases.

关 键 词：骨髓基质细胞 大鼠 

分 类 号：Q813.1[生物学—生物工程]