刚果红共振光散射法测定蛋白质  

Determination of protein based on protein-congo red system by resonance light scattering method

作　　者：王小红[1] 李树伟[1] 苏小东[1] 潘建章[1] 何艺[1] 

机构地区：[1]四川师范大学化学学院,四川成都610066

出　　处：《化学研究与应用》2004年第5期669-670,共2页Chemical Research and Application

摘　　要：In the Britton-Robinson buffer solution at pH=4.35 Congo red combines with protein by intermolecular forces,which resultes in an resonance light scattering(RLS).The RLS intensity reaches a maximum at the wavelength of 575 nm and is proportional to the concentration of protein.The method is simple and rapid with a linear range up to 8.0 μg/mL and a detection limit of 0.054 μg/mL.This method was applied to the determination of human serum protein and compared to Bradford method with a satisfactory result.In the Britton-Robinson buffer solution at pH=4.35 Congo red combines with protein by intermolecular forces,which resultes in an resonance light scattering(RLS).The RLS intensity reaches a maximum at the wavelength of 575 nm and is proportional to the concentration of protein.The method is simple and rapid with a linear range up to 8.0 μg/mL and a detection limit of 0.054 μg/mL.This method was applied to the determination of human serum protein and compared to Bradford method with a satisfactory result.

关 键 词：刚果红 共振光散射 蛋白质 

分 类 号：O657.39[理学—分析化学]