针灸对去卵巢大鼠骨组织TGF-β_1 mRNA、VEGF mRNA表达及凋亡基因Fas的影响  

Effects of Acupuncture and Moxibustion on TGF-β1 mRNA. VEGF mRNA Expression and Apoptosis Gene Fas in Bone Tissue of the Ovariectomized Rats

作　　者：刘献祥[1] 沈霖[2] 吴明霞[3] 吴炳煌[1] 高兰[2] 胡文胜[2] 张安桢[1] 

机构地区：[1]福建中医学院,福州市五四路282号,350003 [2]华中科技大学同济医学院 [3]福建省第二人民医院

出　　处：《中医杂志》2003年第11期830-832,共3页Journal of Traditional Chinese Medicine

基　　金：福建省自然科学基金(C01100l9);福建省教育厅科技资助项目(JA02235)

摘　　要：目的:探讨针灸对去卵巢大鼠骨质疏松症的作用机制和环节。方法:骨组织TGF-β_1 mRNA、VEGF mRNA采用原位杂交,凋亡基因Fas采用免疫组织化学检测。结果:模型组TGF-β_1、VEGF光度低于假手术组(P<0.05),针刺组、艾灸组、雌二醇组TGF-β_1、VEGF光度高于模型组(P<0.05);骨组织FAS蛋白光度,模型组高于假手术组(P<0.01),针刺组、艾灸组和雌二醇组低于模型组(P<0.05～0.01);成骨细胞内FAS蛋白阳性表达率模型组高于假手术组(P<0.01),雌二醇组低于模型组(P<0.01);破骨细胞内FAS蛋白阳性表达率针刺组、艾灸组和雌二醇组高于假手术组(P<0.05～0.01)。结论:针灸能有效调节破骨细胞内Fas基因的表达;能激活TGF-β_1、VEGF活性,使TGF-β_1 mRNA、VEGF mRNA表达增加。Objective: To explore the mechanism of acupuncture and moxibustion for treatment of osteoporosis in ovariectomized rats. Methods: TGF-β1, mRNA. VEGF mRNA expression of bone tissue and apoptosis gene Fas were detected respectively by in situ hybridization and immunohistochemistry. Results: TGF-β1 and VEGF gray density in the model group were lower than those in the sham operation group (P<0. 05), and those in the acupuncture group, the moxibustion group and the estradiol group were higher than those in the model control group (P< 0. 05). Fas protein positive expression rate in osteoblast in the model group was ligher than that in the sham operation group (P<0. 01). and in the estradiol group was lower than that in the model group (P<0. 01). Fas protein positive expression rate in the osteoclast in the acupuncture group, moxibustion group and estradiol group were higher than those in the sham operation group (P < 0.05 - 0.01). Conclusion: Acupuncture and moxibustion can effectively regulate Fas gene expression in osteoblast. stimulate activities of TGF-β1 and VEGF, and increase expression of TGF-β1, mRNA and VEGF mRNA.

关 键 词：骨质疏松症 针灸 大鼠 基因表达 凋亡基因Fas 

分 类 号：R245[医药卫生—针灸推拿学]