同时应用近场光学显微镜的透射和反射模式研究单个细胞的光学性质  

Research on Optical Properties of Single Cells Simultaneously Using Transmission and Reflection Mode of Near-field Scanning Optical Microscopy

作　　者：陈勇[1] 蔡继业[1] 肇静娴[2] 

机构地区：[1]暨南大学生命科学技术学院 [2]暨南大学组织移植与免疫教育部重点实验室,中国广东广州510632

出　　处：《激光生物学报》2003年第6期426-430,共5页Acta Laser Biology Sinica

基　　金：国家重点基础研究发展规划项目("973")基金资助(2001CB510101);教育部科技重点项目(00124);国家自然科学基金(60278014);国家自然科学基金重点项目(30230350)。

摘　　要：通常认为,在近场光学显微技术的光收集模式中,观察透光性好的样品时采用透射模式,研究不透明样品时采用反射模式。本文同时采用透射和反射两种模式观察透明性较好的PC12细胞和淋巴细胞样品,初步研究单个细胞的反射、吸收、透射和荧光等光学性质,以促进组织光学和激光生物医学等领域的进一步发展。细胞光学的时代就要到来。In general, there are two types of near-field scanning optical microscopy, transmission mode and reflection mode. It was thought that, for the penetrable sample by light, transmission mode is used, and for the opaque sample to light, reflection mode is employed. In this paper, both PC12 cell and lymphocyte were observed by near-field scanning optical microscopy, at both transmission mode and reflection mode, to study optical properties of cells, such as reflection, absorbency, transmission, fluorescence, and so on, in order to facilitate the development of tissue optics and laser biomedicine. The era of cell optics will be coming.

关 键 词：PC12细胞 淋巴细胞 近场光学显微镜(NSOM) 透射模式 反射模式 

分 类 号：O436[机械工程—光学工程]