实验性脾虚痹证的黏膜免疫学机制研究  

Studies on Functions of Mucosal Immune in Collagen-induced Arthritis Superimposed on Spleen Deficiency in Mice

作　　者：徐世杰[1] 肖诚[1] 周桂琴[1] 吕爱平[1] 王安民[1] 

机构地区：[1]中国中医研究院基础理论研究所,北京100700

出　　处：《中国中医基础医学杂志》2003年第12期35-38,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目 (3 0 0 70 90 6);国家中医药管理局资助项目 (2 0 0 0 J P 0 7)

摘　　要：目的 :从黏膜免疫及炎症相关细胞因子的角度探讨脾虚痹证的发病机理。方法 :选用Ⅱ型胶原诱导的小鼠关节炎 (CIA)作为痹证模型 ;复合大黄泻下加饥饱失常造成实验动物脾虚 ;以酶联免疫吸附法测定小鼠血清中肿瘤坏死因子 α、白细胞介素 6、干扰素 γ、白细胞介素 10等细胞因子含量 ,以免疫组织化学方法测定小肠组织中CD4、CD8、分泌型IgA含量 ,并对肠道集合淋巴结(PEYER SPATCH ,PP结 )计数。结果 :脾虚组、脾虚痹证组肠道PP结数量明显减少 ,CIA组变化不明显。CIA组及脾虚痹证组血清中TNF α、IL 6含量显著升高 ,CIA组及脾虚痹证组血清中IFN γ含量显著升高 ;与空白对照组比较 ,CIA组、脾虚痹证组CD4、CD8阳性细胞均明显减少 ,而以脾虚痹证组CD8阳性细胞减少更为明显 ;正常组、CIA组SIgA阳性细胞较多 ,脾虚组、脾虚痹证组则明显减少。结论 :脾虚时可能是通过下调机体黏膜免疫功能 。Aims:to explore the pathogenesis of Bi-syndrome(arthritis) superimposed on spleen deficiency by investigating the changes of mucosal immune functions and inflammation-related cytokines.Methods:Arthristis was induced by type Ⅱ collagen (CIA) superimposed on spleen deficiency in DBA mice.Enzyme-linked immunoadsorbent assay (ELISA) was employed to detect the levels of tumor necrosis factor-α,interleukin-6,interferon-γ and interleukin-10 in serum,and immunohistochemistry methods was used to assay CD\-4,CD\-8 and secretory immunoglobulin A in the tissues of small intestine.The numbers of intestinal PEYER'S PATCH(PP) was calculated.Results:the number of intestinal PP apparently reduced in mice with spleen deficiency and in mice with spleen-deficiency-CIA,while there was no significant changes in CIA mice.The leovels of TNF-α\,IL-6 in serum increased significantly in CIA mice and in spleen-deficiency-CIA mice.The content of IFN-γ in serum highly increased while IL-10 decreased in CIA and spleen-deficiency-CIA mice.Compared to that in controlled mice,CD4+ and CD8+ Cells reduced in CIA and spleen-deficiency-CIA mice.SigA positive cells were more in normal and CIA mice,while it reduced apparently in spleen-deficiency and spleen-deficiency-CIA mice.Conclusion:spleen-deficiency may exacerbate the CIA by down-regulate mucosal immunofunctions and relatively up-regulate general immune responses.

关 键 词：睥虚痹证 Ⅱ型胶原诱导性关节性 类风湿性关节炎 黏膜免疫 

分 类 号：R392.3[医药卫生—免疫学]