鸡源霉形体PCR检测方法的研究  

Study on PCR detection about chook mycoplasma

作　　者：牛建强[1] 王希东[2] 党荣理[1] 叶志远[1] 喻梅辉[2] 

机构地区：[1]新疆军区联勤部卫生防疫大队,乌鲁木齐830002 [2]新疆农业大学农学院,乌鲁木齐830052

出　　处：《新疆农业大学学报》2003年第4期76-77,共2页Journal of Xinjiang Agricultural University

基　　金：兰州军区医药卫生资助项目 (XH - 980 16 )

摘　　要：据基因库中鸡源霉形体 16SrRNA基因序列 ,设计了一对跨幅为 10 2 6bp的引物 ,用这对引物对致病性鸡源霉形体MG ,MS的DNA进行PCR扩增 ,结果扩增片段大小与设计相一致 ,而其它细菌扩增结果则均为阴性。We have searched the sequence about chook mycoplasma's 16S rRNA from gene bank, designed the primer about chook mycoplasma's 16S rRNA, the distance between the primer is 1026bp.And we amplified the DNA of MG and MS which can cause the disease by PCR, the size of the PCR product accords with the design, the PCR product of other bacteria's DNA are negative.

关 键 词：鸡源霉形体 PCR 

分 类 号：Q93331[生物学—微生物学]