过氧化物酶同工酶在黑蛋巢菌属分类上的应用研究  

Study on the Roles of Peroxidase Isoenzymes in Classification of the Genus Cyathus

作　　者：余静[1] 王杰[1] 陈玉惠[2] 周彤燊[2] 叶锋平[1] 

机构地区：[1]成都军区联勤部军事医学研究所,云南昆明650203 [2]西南林学院保护生物学学院,云南昆明650224

出　　处：《西南林学院学报》2003年第4期17-20,共4页Journal of Southwest Forestry College

基　　金：国家自然科学基金资助项目(F063).

摘　　要：采用电泳方法分析过氧化物酶同工酶在黑蛋巢菌属种级分类中的意义.结果表明:组间过氧化物酶同工酶相似系数集中在0.20～0.40;种间相似系数多在0.30～0.57;同一个种不同菌株之间相似系数为0.89～1.00,且过氧化物酶同工酶不受地理种源的影响.鸟巢菌科黑蛋巢菌属过氧化物酶同工酶具有种的稳定性,而在组间、组内种间差异较明显,对该属真菌的分类和疑难标本鉴定有一定意义.The roles of peroxidase isoenzymes in classification of genus Cyathus were studied by electrophoresis. It was showed by the results that the similarity coefficient(S_c) of zymograms among groups of peroxidase isoenzyme was 0.20～0.40, and the S_c among different species was 0.30～0.57. It was also indicated that the peroxidase enzymes had less to do with geological origins of the isolates because the S_c reached 0.89～1.00. The results showed that the peroxidase isoenzymes of genus Cyathus was quite stable in the species while the zymogram dissimilarity among the groups and among species in a group was obvious. Therefore it was proven that the peroxidase enzymes might play an important role in classification of this genus.

关 键 词：过氧化物酶同工酶 黑蛋巢菌属 电泳 种间相似系数 组间相似系数 

分 类 号：Q554.6[生物学—生物化学]