家兔实验性动脉粥样硬化血管重塑的形态定量分析  

Quantitative morphometric analysis of vascular remodeling in experimental atherosclerotic rabbits

作　　者：任彩霞[1] 常青[1] 李建芝[1] 唐海兰[1] 黄华梅[1] 李自成[2] 关洁宾[3] 

机构地区：[1]暨南大学医学院组织学与胚胎学教研室,广东广州510632 [2]暨南大学医学院第一附属医院心内科,广东广州510630 [3]暨南大学医学院动物所,广州510632

出　　处：《中国临床解剖学杂志》2003年第3期254-256,268,共4页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金资助项目 (3980 0 0 68)

摘　　要：目的 :探讨AS血管重塑的几何形态变化规律。方法 :将 40只新西兰雄性家兔随机等分为正常对照组 (C组 )和动脉粥样硬化模型组 (AS组 ) ,分别于实验的第 4、8、12周末取各组家兔的胸主动脉进行定性观察及定量分析。结果 :随时间延长 ,AS组斑块面积逐渐增大 ,斑块检出率逐渐增加。管腔面积在早期斑块形成时并无改变 ,晚期则明显缩小。斑块面积及管腔面积均分别与内弹性膜包围面积 (IELA)、外弹性膜包围面积 (EELA)呈正相关关系 (P <0 .0 5 ) ,IELA和EELA间也呈显著正相关关系 (P <0 .0 1) ,但斑块面积与管腔面积间并无直线相关关系 (P >0 .0 5 )。结论 :家兔AS病变随时间延长逐渐加重 ,且在斑块形成时伴内弹性膜和外弹性膜的同时扩张 ,管腔狭窄与否主要与IELA和EELA相关。IELA和EELA可作为判断管腔狭窄及评价血管重塑的指标。Objective: To investigate the morphometric changes of vascular remodeling. Methods: 40 male New Zealand rabbits were divided randomly and equally into two groups: C group (control group) and AS group (atherosclerosis model group). Thoracic aorta from each group were collected to be observed qualitatively and analyzed quantitatively at the end of the fourth, eighth and twelfth week. Results: The plaque area and percentage of plaque formation increased gradually with the proceeding of experiment in AS group. Lumen area varied rarely in early plaque formation, while decreased significantly at the advanced stages. Meanwhile, both plaque area and lumen area correlated positively with internal elastic laminal area (IELA) and external elastic laminal area (EELA) ( P <0.05). IELA correlated positively with EELA, too ( P >0.05). Conclusions: The changes of lumen area correlate mainly with IELA and EELA but not with plaque area. IELA and EELA can be seen as the parameters to judge lumen stenosis and to evaluate vascular remodeling.

关 键 词：动脉粥样硬化 血管重塑 形态定量分析 

分 类 号：R543[医药卫生—心血管疾病]