皮肌炎/多发性肌炎患者白介素4、干扰素γ、转化生长因子β1的检测  

Detection of IL-4、IFN-γ、and TGF-β1 in Patients with Dermatomyositis/Polymyositis

作　　者：陈爱明[1] 刘青[1] 钱伯源[1] 彭群新[2] 吴昀[2] 

机构地区：[1]苏州大学附属第一医院皮肤科,215006 [2]苏州大学附属第一医院检验科,215006

出　　处：《中华皮肤科杂志》2003年第9期505-506,共2页Chinese Journal of Dermatology

摘　　要：目的探讨皮肌炎(DM)/多发性肌炎(PM)的Th1/Th2/Th3细胞功能性极化。方法采用酶联免疫吸附试验(ELISA)测定23例DM/PM患者(DM18例,PM5例)和17例健康人的外周血单一核细胞(PBMC)培养上清液中白介素4(IL-4)、干扰素γ(IFN-γ)、转化生长因子(TGF)-β1的含量。结果DM/PM患者的IFN-γ水平低于健康对照组(P<0.02),IL-4和TGF-β1分别高于健康对照组(P值均<0.05),IFN-γ/IL-4比值小于对照组(P<0.02)。在肺X线片或CT片上,DM10例、PM1例示肺纹理增多、增粗或纤维化,其TGF-β1分别高于无肺纹理增多、增粗或纤维化的患者(DM6例,PM2例)(P>0.05)和健康对照组(P<0.05)。结论DM/PM患者的Th1/Th2/Th3型细胞因子网络出现异常,IL-4和TGF-β增高,IFN-γ下降。Objective To investigate the polarization of Th1/Th2/Th3cell function of dermatomyositis/polymyositis(DM/PM).Methods The culture supernatant levels of IL-4,IFN-γ,TGF-β1that were produced by the peripheral blood mononuclear cells(PBMC)were detected by Sandwich ELISA in23DM/PM patients(DM18,PM5)and17healthy subjects.Results The level of IFN-γ(168.17±218.35ng/mL)produced by PBMC from the patients of DM/PM was significantly lower than that from normal controls(380.61±299.13ng/mL)(P<0.02).The levels of IL-4(34.68±11.12ng/mL)and TGF-β1(332.93±196.99ng/mL)were significantly increased,compared with those from normal controls(IL-4:27.95±7.55ng/mL,TGF-β1:231.64±83.92ng/mL)(P<0.05).The ratio of IFN-γto IL-4(5.05±6.25)was significantly smaller than that of normal controls(14.29±12.59)(P<0.02).Lung X-ray examination or CT was performed in19DM/PM patients(DM16,PM3).Pulmonary fibrosis or a lot of increased lung marking on CT or pneumonograph was shown in eleven patients(DM10,PM1).The TGF-β1level in these patients was significantly higher than those(DM6,PM2)without pulmonary fibrosis or increased lung marking(P>0.05),and normal controls(231.64±83.92)(P<0.05).Conclusions There is imbalance of Th1/Th2/Th3cytokines in of the patients with DM/PM:levels of IL-4and TGF-βare higher with lower IFN-γthan those in normal controls.

关 键 词：皮肌炎 多发性肌炎 酶联免疫吸附试验 白介素4 干扰素Γ 转化生长因子Β1 检测 

分 类 号：R593.26[医药卫生—内科学]