转甜菜碱醛脱氢酶基因水稻的获得及其耐盐性研究  

Production and Salt-tolerant Research of Transgenic Rice with Betaine Aldehyde Dehydrogenase Gene

作　　者：卢德赵[1] 王慧中[2] 华志华[3] 颜美仙[3] 钱前[3] 黄大年[3] 

机构地区：[1]浙江中医学院生命科学系,浙江杭州310053 [2]杭州师范学院生物化学与分子生物学重点实验室,浙江杭州310036 [3]中国水稻研究所农业部水稻生物学重点实验室,浙江杭州310006

出　　处：《科技通报》2003年第3期179-182,共4页Bulletin of Science and Technology

基　　金：国家自然科学基金(No.39970409);中国水稻科学发展基金(No.0003239);浙江省自然科学基金(No.302061)资助项目

摘　　要：利用农杆菌介导法和基因枪法将来源于山菠菜的甜菜碱醛脱氢酶基因(BADH)转入水稻基因组,并获得了具有一定耐盐性状的转基因植株,在0.5%NaCl盐浓度下生长良好,同时对Kan和G418的筛选效果及两种转化方法进行了比较.Betaine aldehyde dehydrogenase gene (BADH) was transferred into rice genome by Agrobacteriummediated and particle bombardment, and 97 transgenic plants were obtained. Those transgenic plants grew well while the control was stagnated when treated with 0.5%NaCl. Simultaniously, comparisons were made between the selective effects of Kan and G418, and between the two transformation methods, respectively. 

关 键 词：遗传学 甜菜碱醛脱氢酶基因 基因转化 耐盐性 

分 类 号：Q785[生物学—分子生物学] S330[农业科学—作物遗传育种]