评价三种检测AmpC酶的方法及临床应用  

Evaluation and clinical application three mechods for detection of AmpCβ-lactamases

作　　者：陈俐丽[1] 周铁丽[2] 

机构地区：[1]温州医学院检验医学专业98年级,浙江温州325027 [2]温州医学院附属第一医院检验科,浙江温州325000

出　　处：《检验医学教育》2003年第3期42-44,共3页

摘　　要：目的:比较三种检测AmpC酶的方法,以找出一种能简便、准确、适合临床常规应用的检测AmpC酶的方法。同时了解我院临床分离菌株AmpC酶的产生情况。方法:采用酶提取物三维试验、头抱西丁三相试验及氯唑西林双纸片协同法同时检测75株阴沟肠杆菌和55株鲍曼不动杆菌的AmpC酶产生情况。结果:用酶提取物三维试验检测到所试阴沟肠杆菌和鲍曼不动杆菌的AmpC酶阳性株分别为22株(占29.3%)和10株(占18.2%)、头孢西丁三相试验及氯唑西林双纸片协同法与酶提取物三维试验的符合率分别为70.8%(92/130)和94.6%(123/130)。结论:头孢西丁三相试验检测AmpC酶存在严重的假阳性,而氯唑西林双纸片协同法结果可靠、方法简便,适合于临床常规应用。objective:Compare three methods for detection of AmpCβ-lactamases in order to find a simple and credible method routinely use in the clinical microbiology laboratory, and determined the prevalence in our hospital of clinical strains producing AmpCs. Methods: AmpCs was detected by three dimensional test, Cefoxitin 3-dimensional test,diffusion synergy test in E. cloacae and A. baumanii. Results:Among 75strains of E. cloacae, 55 strains of A. baumanii, producing AmpCs strains accounted for 22 (29.3%), 10 (18.2%), compared with three dimensional test,the coincidence rate of cefoxitin 3-dimensional test, diffusion synergy test were 70.8% (92/130) and 94.6(123/130) respectively. Conclutions:Detect AmpCs using Cefoxitin 3-dimensional test may obtain false positive. Diffusion synergy test simple and credible,and is suitable for routine application.

关 键 词：AMPC酶 检测方 酶提取物三维试验 头孢西丁三相试验 氯唑西林双纸片协同法 

分 类 号：R446.1[医药卫生—诊断学]