The Karyotypes, C-banding Patterns and AgNORs of Epinephelus malabaricus  

作　　者：邹记兴  Hu Chaoqun  Xiang Wenzhou  YU Qixing  Zhou Fei  

机构地区：[1]South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, P.R. China [2]College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China

出　　处：《High Technology Letters》2004年第2期1-4,共4页高技术通讯（英文版）

基　　金：中国科学院资助项目

摘　　要：The chromosome specimens of Epinephelus malabaricus (Bloch & Schneider, 1801) are obtained from metaphase of kindney cell by vivi-injection of PHA and culture of colchicines, hypatoic-air drying technique, and then by studying their Giemsa stain, C-bands and AgNORs. The results are as follows: (1)E. malabaricus has a diploid chromosome number of 48 and its karyotype formula is 48t, NF=48, sex chromosome is not found. (2) There is a pair of chromosomes with secondary constriction near the centromere of chromosome t24. (3) 1～4 nucleoli appear in the nucleus of interphase, 55% nuclei has 1 nucleolus and only 2% for 4 nucleoli. (4) AgNORs appear in the chromosome t24 of 50% metaphase, sometimes in the chromosome t5, but not in other chromosomes. (5) The AgNORs polymorphisms are individually specific, 1～4 pairs of the number, and the frequency of 4 AgNORs are lowest. (6) The secondary constrictions and positive C-bands are coincident, close to the centromere of the chromosome, and mass constrictive heterochromatins appear in that region. (7) All the centromeres of chromosomes are darkly stained C-bands, and the whole arm of chromosome t24 and its centromere are same positive C-bands. (8) The evolutive regulation of the karyotype and the developing mechanism of AgNORs and C-bands are discussed.

关 键 词：染色体组型 C-带 黑带石斑鱼 鱼类 AGNORS 

分 类 号：Q959.4[生物学—动物学] Q343.2