光敏生物素标记淋球菌基因探针的实验研究  

Preparation Gonococcal Gene DNA Probe Labeled with Photobiotin

作　　者：马佳毓[1] 郭晓奎[1] 韩淑兰[1] 赵月辉[1] 李绍贤[1] 王新春[2] 于成业 陈艳云 李志文[3] 相佳林 孙延增 

机构地区：[1]哈尔滨医科大学微生物学教研室 [2]哈尔滨医科大学二院 [3]黑龙江省性病防治中心

出　　处：《哈尔滨医科大学学报》1993年第4期305-307,共3页Journal of Harbin Medical University

摘　　要：本文用碱变性法提取淋球菌92023的DNA,经光敏生物素标记制成基因探针。此探针与8株淋球菌的DNA全部杂交阳性,而不与大肠杆菌、绿脓杆菌、嗜乳糖奈瑟菌等其它细菌的DNA杂交。对36份临床标本的检测结果表明,23份结果阳性,13份阴性。细菌分离培养方法结果是22份阳性,14份阴性。两方法符合率为97,22%。说明本文探针是一种特异、敏感性良好,适合临床使用的新技术。The gene DNA for preparating probe isolated from N. gonorrhoeae 92023 by alkaline lysis method and labeled with photobiotin. The probe hybridized only with 8 strains N, gonorrhoeae DNA, but not with E. coli, P.aeruginosa, N.lactamica and other bacteria DNA by dot blot assay. The detection of 36 clinical samples.with this probe revealed that sensitivity was 22/22(100%) and specificity was 22/23 ( 95.65%).Therefore, the probe was thought to be specific and sensitive.

关 键 词：基因探针 光敏生物素 淋病奈氏球菌 

分 类 号：R759.204[医药卫生—皮肤病学与性病学]