Molecular characterization of RA68 related potentially to rice flower development  

作　　者：WUXiaohuai WANGTai 

机构地区：[1]KeyLaboratoryofPhotosynthesisandEnvironmentalMolecularPhysiology,InstituteofBotany,ChineseAcademyofSciences,Beijing100093,China

出　　处：《Chinese Science Bulletin》2004年第14期1476-1480,共5页

摘　　要：One cDNA whose corresponding mRNA was preferentially accumulated in rice flowers was isolated by PCR-mediated RNA subtraction hybridization and RACE strategy. This gene, termed RA68, consists of three exons and two introns. the putative RA68 protein contained 219 amino acid residues with a putative signal peptide and two domains. The N-terminal domain is hydrophilic and rich in proline and threonine embedded in PTPTSYG motifs. The C-terminal domain is hydrophobic. Southern blot and se-quence analysis reveal that RA68 exists as one copy in the rice genome and localizes in chromosome 2. Northern hy-bridization indicates that RA68 is expressed preferentially in buds and flowers, but not in roots and leaves. Furthermore, in situ hybridization indicates that RA68 transcripts are pre-sent in pollen mother cells (PMCs), dyad cells, uninucleate pollens and macrospore sacs. Possible functions of RA68 dur-ing the flower development are discussed based on its expression and protein structure characteristics.One cDNA whose corresponding mRNA was preferentially accumulated in rice flowers was isolated by PCR-mediated RNA subtraction hybridization and RACE strategy. This gene, termed RA68, consists of three exons and two introns. the putative RA68 protein contained 219 amino acid residues with a putative signal peptide and two domains. The N-terminal domain is hydrophilic and rich in proline and threonine embedded in PTPTSYG motifs. The C-terminal domain is hydrophobic. Southern blot and se-quence analysis reveal that RA68 exists as one copy in the rice genome and localizes in chromosome 2. Northern hy-bridization indicates that RA68 is expressed preferentially in buds and flowers, but not in roots and leaves. Furthermore, in situ hybridization indicates that RA68 transcripts are pre-sent in pollen mother cells (PMCs), dyad cells, uninucleate pollens and macrospore sacs. Possible functions of RA68 dur-ing the flower development are discussed based on its expression and protein structure characteristics.

关 键 词：RNA减数杂交 RA68 水稻花 原位杂交 ORYZA SATIVA 

分 类 号：S511[农业科学—作物学] S334