Transfer of bacterial blight resistance from Oryza meyeriana to O. sativa L. by asymmetric somatic hybridization  

作　　者：ZHUYongsheng CHENBaotang YUShunwu ZHANGDuanpin ZHANGXueqin YANQiusheng 

机构地区：[1]NationalKeyLaboratoryofCropGeneticImprovement,HuazhongAgriculturalUniversity,Wuhan430070,China [2]ChinaNationalRiceResearchInstitute,Hangzhou310006,China

出　　处：《Chinese Science Bulletin》2004年第14期1481-1484,共4页

摘　　要：Asymmetric somatic hybrid plants were pro-duced between cultivated rice (Oryza sativa L.) and wild spe-cies [O. meyeriana (Zoll. etMor. exSteud.)] with high resis-tance to rice bacterial blight. X-ray-irradiated protoplasts of the wild species were used as donor and chemically fused with iodoacetamide-inactivated protoplasts of rice cv. 02428 to produce hybrids. Seventy-two plants were regenerated from 623 calli based on metabolic complementation. The morphological characters of the plants closely resembled that of the rice. Simple sequence repeats were employed to iden-tify their hybridity. Cytological analysis of root-tips revealed that their chromosome number varied in the range of 27— 38. The somatic hybrids were inoculated with strains of Xanthamonas oryzae pv. oryzae at adult growth stage and demonstrated the resistance to bacterial blight introgression from the O. meyeriana.Asymmetric somatic hybrid plants were pro-duced between cultivated rice (Oryza sativa L.) and wild spe-cies [O. meyeriana (Zoll. etMor. exSteud.)] with high resis-tance to rice bacterial blight. X-ray-irradiated protoplasts of the wild species were used as donor and chemically fused with iodoacetamide-inactivated protoplasts of rice cv. 02428 to produce hybrids. Seventy-two plants were regenerated from 623 calli based on metabolic complementation. The morphological characters of the plants closely resembled that of the rice. Simple sequence repeats were employed to iden-tify their hybridity. Cytological analysis of root-tips revealed that their chromosome number varied in the range of 27— 38. The somatic hybrids were inoculated with strains of Xanthamonas oryzae pv. oryzae at adult growth stage and demonstrated the resistance to bacterial blight introgression from the O. meyeriana.

关 键 词：体细胞杂交 水稻细菌疫病 ORYZA SATIVA L. ORYZA 水稻 代谢互补 

分 类 号：S511[农业科学—作物学] S334