Function of A-Rich region in DNAβassociated with Tomato yellow leaf curl China virus  

作　　者：TAOXiaorong QINGLing ZHOUXueping 

机构地区：[1]InstituteofBiotechnology,ZhejiangUniversity,Hangzhou310029,China

出　　处：《Chinese Science Bulletin》2004年第14期1490-1493,共4页

摘　　要：A novel type of circular single-stranded satellite DNA, known as DNAb, was recently characterized and demonstrated to be associated with monopartite begomovi-ruses. DNAb was essential for induction of characteristic symptoms in plants. DNAb has three structural features: an 115 bp highly conserved region, bC1 gene and A-Rich region. The in-frame ATG mutation of bC1 gene of Tomato yellow leaf curl China virus isolate Y10 (TYLCCNV-TY10) DNAb demonstrated that bC1 gene is required for leaf curl symptom. Here, the function of A-Rich region in TYLCCNV-Y10 DNAb was identified. When A-Rich region was deleted, the A-Rich deleted mutant was still capable of replication and systemic infection in plant, indicating that A-Rich region is not required for trans-replication of DNAb. The immunotrapping-PCR demonstrated that A-Rich de-leted mutant could be encapsidated in the coat protein en-coded by TYLCCNV-Y10 DNA-A, suggesting that A-Rich region is not related with DNAb encapsidation. However, the A-Rich region deleted mutant caused milder symptom.A novel type of circular single-stranded satellite DNA, known as DNAb, was recently characterized and demonstrated to be associated with monopartite begomovi-ruses. DNAb was essential for induction of characteristic symptoms in plants. DNAb has three structural features: an 115 bp highly conserved region, bC1 gene and A-Rich region. The in-frame ATG mutation of bC1 gene of Tomato yellow leaf curl China virus isolate Y10 (TYLCCNV-TY10) DNAb demonstrated that bC1 gene is required for leaf curl symptom. Here, the function of A-Rich region in TYLCCNV-Y10 DNAb was identified. When A-Rich region was deleted, the A-Rich deleted mutant was still capable of replication and systemic infection in plant, indicating that A-Rich region is not required for trans-replication of DNAb. The immunotrapping-PCR demonstrated that A-Rich de-leted mutant could be encapsidated in the coat protein en-coded by TYLCCNV-Y10 DNA-A, suggesting that A-Rich region is not related with DNAb encapsidation. However, the A-Rich region deleted mutant caused milder symptom.

关 键 词：番茄黄叶卷曲中国病毒 DNAΒ A-富集区 双生病毒 功能 

分 类 号：S436.412.1[农业科学—农业昆虫与害虫防治]