VTA微量注射溴隐亭诱发大鼠睡眠——觉醒的改变  

Changes of Sleep—wakefulness Induced by Microinjection of Bromocriptine Into the Ventral Tegmental Area in Rats

作　　者：钱朝霞[1] 林殷利[2] 

机构地区：[1]海南医学院生理教研室,海口570005 [2]上海第二医科大学生理教研室

出　　处：《海南大学学报（自然科学版）》1993年第3期61-66,共6页Natural Science Journal of Hainan University

摘　　要：实验在15只清醒的SD雄性大鼠上完成。利用多巴胺受体激动剂——溴隐亭进行双侧中脑腹侧被盖区(VTA)微量注射,实验观察了四种不同的剂量(5μg/0.5μl、2.5μg/0.5μl、1μg/0.5μl、0.5μg/0.5μl)。结果表明:(一)5μg/0.5μl、2.5μg/0.5μl的溴隐亭注射后,第2～3h的觉醒(W)时间分别增加9.7min(P<0.05)及15.5min(P<0.05),慢波睡眠(SWS)时间分别减少13.3min(p<0.05)及15.2min(P<0.05),异相睡眠(PS)时间无显著变化(P>0.05)。W、SWS、PS的次数无显著性变化。(二)1μg/0.5μl、0.5μg/0.5μl的溴隐亭注射后,第2～3h的W、SWS、PS的时间和次数均无显著性变化。上述结果提示:双侧VTA微量注射溴隐亭可致大鼠觉醒增加、慢波睡眠减少,但无双相作用。The experiments were carried out to in 15 conscious male SD rats. Bromocriptine, a dopaminergic agonist, was micro—injected into the ventral tegmental area (VTA) of the brain, four different dosages to introduce as following: 5μg/0.5μl, 2.5μg/0.5μl, 1μg/0.5μl and 0.5μg/0.5μl. When bromocriptine was injected in first two dosages (5μg/0.5μl and 2.5μg/0.5μl) it was seen that during the second and the third hour after injection the amount of waking (W) increased for 9.7 min (bromo—:33.8±3.4min, control:24.1±3.0min) and 15.5 min (bromo—:39.7±5.6min,control:24.2±2.5min) respectively (P<0.05), while the amount of slow wave sleep (SWS) decreased for 13.3 min (bromo—:78.4±3.2min, control:91.7±3.0min) and 15.2 min (bromo—:74.5±5.0min, control:89.7±8.3 min) respectively (P<0.05). But the amount of paradoxical sleep (PS) had no change (P>0.05). The times of W, SWS and PS did not change significantly (P>0.05). When the bromocriptine were given in last two dosages (1μg/0.5μl and 0.5μg/0.5μl), there were no significant changes in all parameters measured (P>0.05). The experimental results showed that the microinjection of bromocriptine into the VTA could influence the amount of W and SWS. But it had no biphasic effects.

关 键 词：溴隐亭 VTA 多巴胺 诱发 睡眠 觉醒 

分 类 号：R338.63[医药卫生—人体生理学]