石菖蒲挥发油对癫痫大鼠海马氨基酸含量的影响  

Changes of amino acid content in hippocampus of epileptic rats treated with volatile oil of Acorus tatarinowii

作　　者：陈俐[1] 廖卫平[2] 

机构地区：[1]广州医学院第一附属医院神经内科,广东广州510120 [2]广州医学院第二附属医院神经研究所,广东广州510260

出　　处：《中国中药杂志》2004年第7期670-673,共4页China Journal of Chinese Materia Medica

基　　金：广东省医学科研基金资助项目 (A2 0 0 3 2 86)

摘　　要：目的 :研究石菖蒲萃取挥发油对癫痫大鼠海马内兴奋性与抑制性氨基酸含量的影响 ,探讨其抗癫痫的可能机制。方法 :用超临界CO2 萃取法 (SFE CO2 )提取石菖蒲挥发油 ,脑室内注射海人酸 (KA)建立大鼠癫痫模型 ,观察经挥发油治疗后的癫痫大鼠海马氨基酸含量的变化。结果 :经石菖蒲挥发油 35mg·kg-1腹腔注射后的KA大鼠海马的GABA含量明显升高 ,谷氨酸 (Glu)显著降低 (P <0 .0 5 )。结论 :石菖蒲萃取挥发油可调节癫痫大鼠脑内的兴奋性与抑制性氨基酸的平衡 ,从而起到抗癫痫的作用。Objective:To study the changes of excitatory and inhibitory amino acid content in hippocampus of epileptic rats treated with volatile oil of A. tatarinowii, and explore the possible antiepiletic mechanism. Method: The volatile oil was extracted through Supercritical-CO 2 Fluid Extraction (SFE-CO 2), and epileptic models were built up by kainic acid (KA) lateral ventricle injection. The content of amino acid in hippocampus of epileptic rats treated with volatile oil was calculated. Result: The content of GABA increased and Glu decreased prominently (P<0.05) after volatile oil 35 mg·kg -1 intraperitoneal injection. Conclusion: The volatile oil of A. tatarinowii can modulate the balance of excitatory and inhibitory amino acid in epileptic rats, thereby exerting its antiepileptic effect.

关 键 词：石菖蒲挥发油 癫痫 大鼠 海马氨基酸 谷氨酸 GABA 

分 类 号：R285.5[医药卫生—中药学]