干扰素诱导表达的淋巴细胞抗原6复合体E和干扰素诱导蛋白1基因与系统性红斑狼疮患者病情活动性的关系  

Interferon-inducible genes lymphocyte antigen 6 complex E and tetratricopeptide repeats 1 are correlated with clinical features of patients with systemic lupus erythematosus

作　　者：汤建平[1] 顾越英[1] 沈南[1] 叶霜[1] 钱捷[1] 华晶[1] 陈兴国[1] 陈顺乐[1] 鲍春德[1] 王元[1] 张巍[1] 

机构地区：[1]上海第二医科大学附属仁济医院风湿免疫科上海市风湿病研究所,200001

出　　处：《中华医学杂志》2004年第14期1157-1160,共4页National Medical Journal of China

基　　金：国家"八六三"高新技术研究发展规划基金资助项目(2002AA2 2 9101);国家自然科学基金资助项目(30000154;30 27122 4);上海市科技发展基金资助项目(0 1JC14 0 2 9;0 2QMB14 0 4);上海市科学技术委员会科研计划基金资助项目(0 3DJ14 0 0 9)

摘　　要：目的　探讨系统性红斑狼疮 (SLE)患者外周血单个核细胞 (PBMC)中干扰素诱导表达的淋巴细胞抗原 6复合体E(LY6E)基因与干扰素诱导蛋白 1(IFIT1)基因的实时定量表达水平与SLE疾病特异性和病情活动的相关性。方法　收集 14 4例SLE患者、2 7例非SLE患者与 5 9名正常对照人群的临床资料 ,取外周血抽提总RNA并逆转录成cDNA ,运用SYBRgreendyeⅠ实时定量聚合酶链反应 (PCR)在ABIPRISM 790 0H基因测序仪上检测患者组和对照组的LY6E和IFIT1mRNA定量表达水平 (以 -ΔΔCT值表示 )的差异 ,并与病情活动度进行分组比较 ,分析其意义。结果　 ( 1)SLE患者总体的LY6EmRNA -ΔΔCT值 ( 5 5± 2 0 )显著高于非SLE患者 ( 3 4± 1 7,P =0 0 0 0 )和正常人 ( 4 5± 1 6 ,P =0 0 0 1)。 ( 2 )SLE活动组的LY6E和IFIT1的mRNA -ΔΔCT值 ( 6 2± 1 8,6 5±2 6 )均显著高于SLE非活动组 ( 4 4± 1 8,4 1± 2 6 ) (均P =0 0 0 0 )。 ( 3)SLE患者组的LY6E和IFIT1mRNA -ΔΔCT值与SLEDAI积分、符合SLE分类标准的条数之间有相关性 (P <0 0 0 1)。结论　作为干扰素诱导表达的基因 ,LY6EmRNA定量水平的升高对SLE患者的诊断有一定的特异性 ,LY6EmRNA和IFIT1mRNA的实时表达水平对SLE患者的病情活动度判断均有较大价值 。Objective To investigate the expression levels of lymphocyte antigen 6 complex, locus E (LY6E) and interferon induced protein with tetratricopeptide repeats 1 (IFIT1) genes in the peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SLE), and to evaluate the relations between these gene expression levels and disease activity. Methods The clinical data of 144 SLE patients, 27 non SLE patients with rheumatic diseases, and 59 normal controls were collected. The SLE patients were further divided into 2 subgroups: active SLE group ( n =87) and non active SLE group ( n =57) according to SLEDAI scores. Specimens of peripheral blood were drawn; total RNA was extracted and transcribed into cDNA. Sybr green dye based real time quantitative PCR method was used to compare the expression levels (indicated as-ΔΔCT value) of LY6E and IFIT1 in patients with SLE and those in the controls. Results (1) The-ΔΔCT value of LY6E expression level of the SLE patients was 5.4760± 1.9806, significantly higher than those of the non SLE patients(3.4323±1.7456)and normal controls (4.5198± 1.6359, both P =0.001). (2) The-ΔΔCT value of LY6E and IFIT1 mRNA expression of the active SLE patients were 6.1960 ± 1.7729 and 6.4997±2.6297 respectively, significantly higher than those observed in the inactive SLE patients(4.3770±1.7764 and 4.1327±2.6044 respectively, both P =0.000).The -ΔΔCT values of LY6E and IFIT1 mRNA of the SLE patients were correlated with the SLEDAI scores, and with the numbers of matched criteria used in the diagnosis of SLE ( P <0.001). Conclusion As IFN induced genes, elevated expression of LY6E mRNA is specific to diagnosis of SLE. The real time expression levels of LY6E and IFIT1 genes are associated with SLE disease activity. To inhibit the expression of LY6E and IFIT1 may become a novel therapeutic approach for SLE.

关 键 词：干扰素 基因表达 系统性红斑狼疮 SEE 淋巴细胞 抗原6复合体E 诱导蛋白1基因 活动性 聚合酶链反应 

分 类 号：R593.241[医药卫生—内科学]