高糖对大鼠GMC表面β_1整合素表达和ECM分泌的影响  

Effects of high glucose on β1-integrin expression and extracellular matrix secretion in glomerular mesangial cells

作　　者：谈艳[1] 杜勤[1] 盛净[1] 

机构地区：[1]上海第二医科大学第九人民医院老年科,上海200011

出　　处：《上海第二医科大学学报》2004年第8期635-637,666,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市卫生局科研基金资助项目(004L01).

摘　　要：目的 观察高糖对体外培养的大鼠肾小球系膜细胞(GMC)表面β1整合素表达和细胞外基质(ECM)分泌的影响。方法 应用不同浓度的葡萄糖刺激培养GMC,采用MTT法检测细胞增殖,RT-PCR法检测GMC表面β1整合素的表达,ELISA法检测纤维连接蛋白(FN)、层粘蛋白(LM)的分泌情况。结果 高糖抑制GMC的增殖,促进β1整合素的表达,且与FN和LM的分泌呈显著正相关,并具有浓度依赖性。结论 高血糖作为糖尿病肾病的启动因素可能是通过上调β1整合素mRNA的表达,从而促进ECM成分FN和LM的合成分泌。Objective To observe the effects of high glucose on β1 integrin mRNA expression in cultured glomerular mesangial cells ( GMC). Methods Cultured glomerular mesangial cells were treated with glucose in different concentrations. The proliferation of GMC was measured by MTT, the expression of β1 integrin was estimated by RT-PCR, and the secretion of fibronectin and laminin were measured by ELISA, respectively. Results High glucose inhibited the proliferation of GMC and promoted the expression of β1 integrin, which had a significant correlation with the secretion of fibronectin and laminin. The effect was dose-dependent. Conclusion Hyperglycemia, as an initial factor of diabetic nephropathy, may increase the synthesis and secretion of fibronectin and laminin of the extracellular matrix (ECM) through the up-regulation of β1 integrin mRNA expression.

关 键 词：高糖 大鼠 GMC表面 Β1整合素 ECM分泌 肾小球系膜细胞 糖尿病肾病 

分 类 号：R692[医药卫生—泌尿科学]