工频电磁场对体外培养的小鼠骨髓间充质干细胞增殖与分化的影响  

Effects of exposure to electromagnetic fields on proliferation and differentiation of mouse bone marrow mesenchymal stem cells

作　　者：任凯[1] 吴华[1] 赵文春[1] 葛保健[1] 方真华[1] 印卫锋[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院骨科

出　　处：《中华物理医学与康复杂志》2004年第7期385-387,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金资助项目 (No .50 3470 2 5)

摘　　要：目的　研究 5 0Hz工频电磁场对体外培养的小鼠骨髓间充质干细胞的增殖、分化及细胞内环磷酸腺苷 (cAMP)水平的影响。方法　体外培养小鼠骨髓间充质干细胞 ,取第 3代细胞 ,应用工频电磁场及成骨性诱导剂干预 ,并分别于第 3天和第 5天用MTT法检测其增殖活性 ,用放免法检测细胞内cAMP浓度 ,于第 7天检测细胞内碱性磷酸酶 (ALP)的活性。结果　频率 5 0Hz、强度 2mT的正弦波电磁场能抑制体外培养的小鼠骨髓间充质干细胞的增殖 (P <0 .0 5 ) ,使其ALP活性增高 (P <0 .0 5 ) ,在磁场作用的早期 ( 1～ 3d) ,细胞内的cAMP水平明显增高 (P <0 .0 1) ,继续暴磁至第 5天 ,cAMP水平不再发生变化 (P >0 .0 5 )。结论　 5 0Hz工频电磁场可能通过作用于cAMP 蛋白激酶A信号转导系统 ,从而调控体外培养的小鼠骨髓间充质干细胞的增殖与分化 ,此调控作用可能发生在细胞受电磁场作用的早期。Objective To study the effects of electromagnetic fields (EMFs) on proliferation,differentiation and intercellular cyclic AMP (cAMP) in mouse bone marrow mesenchymal stem cells (MSCs) in vitro. Methods The mouse bone marrow mesenchymal stem cells were isolated and cultured in vitro,the passage three cells were divided into A,B,C and D groups and stimulated with electromagnetic fields (EMFs) under different conditions. The cellular proliferation (MTT),differentiation (alkaline phosphatase activity,ALP),and the intercellular cAMP level were investigated at different time points. Results EMFs of 50Hz frequency and 2mT intensity inhibited cellular proliferation ( P <0.05),enhanced cellular differentiation ( P <0.05),and increased the intercellular cAMP level ( P <0.01) in the early stage of the stimulation (1～3 day),the intercellular cAMP level did not change further in the later stage of stimulation. Conclusion Low frequency EMFs might regulate proliferation and differentiation of the mesenchymal stem cells through mediating cAMP-protein kinase A (cAMP-PKA) signal transduction pathway,which occurred in the early period of the stimulation.

关 键 词：工频电磁场 体外培养 细胞分化 小鼠 骨髓间充质干细胞增殖 碱性磷酸酶 

分 类 号：R35[医药卫生—基础医学]