自体骨髓间充质干细胞促进大鼠坐骨神经缺损修复的实验研究  

An Experimental Study of Autologous Mesenchymal Stem Cells Promoting Peripheral Nerve Regeneration in Adult Rats

作　　者：廖文[1] 刘淑红[1] 张玉富[2] 刘慧哲[3] 李章华[2] 赵永歧[1] 范文红[1] 夏仁云[2] 罗永湘[2] 范明[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]华中科技大学同济医学院附属同济医院骨科,武汉430030 [3]河北职工医学院附属医院肌电图室,保定071000

出　　处：《华中科技大学学报（医学版）》2004年第4期459-462,517,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家"十五"863生物技术领域课题基金资助项目(No2001AA216031);北京市二四八工程重大创新基金资助项目(NoH010210190123)

摘　　要：目的　观察自体骨髓间充质干细胞在神经再生室中的作用 ,探讨其作为组织工程的种子细胞修复周围神经缺损的可行性。方法　成年Wistar大鼠 2 4只 ,随机分成 3组 ,每组 8只 ,硅胶导管桥接右侧 13mm的坐骨神经缺损 ,A组在神经再生室内植入骨髓间充质干细胞 +ECM (extracellularmatrix ,ECM )凝胶 ;B组植入ECM凝胶 ;C组注入生理盐水。术后 12周 ,进行大体观察及坐骨神经功能指数测定、电生理检测、神经组织学等观察。结果　A、B两组均有再生神经生成 ,但A组各项检测指标均优于B组 (P <0 0 5或 0 0 1)。结论　自体骨髓间充质干细胞能够促进周围神经再生 ,可以作为种子细胞应用于外周神经组织工程。Objective To evaluate the feasibility of using autologous mesenchymal stem cells as seed cells to repair peripheral nerve defects in a adult rat model. Methods Twenty-four adult Wistar rats were divided into 3 groups equally. A 13 mm-long nerve defect was made in the right sciatic nerve and was bridged by a 16 mm-long silicone tube. The silicone tube was filled with the compound of autologous mesenchymal stem cells and extracellular matrix (ECM) gel for group A, ECM gel for group B and saline for group C. Gross observation, electrophysiological examination, hisotological staining and measurement of nerves sciatic nerve function index were carried out at 12 weeks post-operation. Results At 12 weeks post-operation, regenerated nerve existed at both group A and group B. However, each mesurement index in group A was superior to that in group B (P<0.05 or P<0.01). Conclusion Autologous mesenchymal stem cells can promote peripheral nerve regeneration and can be used as seed cells for nerve tissue engineering.

关 键 词：骨髓间充质干细胞 大鼠 坐骨神经 神经缺损 神经修复 组织工程 

分 类 号：R651.3[医药卫生—外科学]