深低温冻储消减气管抗原的实验研究  

Minimizing the antigenicity of tracheal allografts by using a technique of cryopreservation

作　　者：王连召[1] 周刚[1] 范飞[1] 张锋[1] 薛富善[1] 栾杰[1] 蔡国斌[1] 孟凡会[2] 王沛涛[2] 王化冰[3] 

机构地区：[1]中国医学科学院中国协和医科大学整形外科医院瘢痕综合治疗中心,北京100041 [2]青岛大学医学院附属医院 [3]卫生部北京医院

出　　处：《中华整形外科杂志》2004年第4期297-300,共4页Chinese Journal of Plastic Surgery

摘　　要：目的　研究深低温冻储消减气管抗原 ,用于同种异体移植的可行性及所需冻储的最短时间。方法　通过纤维支气管镜检查、黏膜血流量的测量、病理学检查、血管造影以及存活率和通畅度的计算 ,分别研究以犬单侧胸头肌瓣和双侧胸骨舌骨肌 胸骨甲状肌联合瓣包裹自体移植气管和未冻储、冻储不同时间的同种异体移植气管术后的存活情况。结果　未冻储和冻储 1d ,2、4周的同种异体气管 ,移植后第 1周时黏膜外观以及黏膜血流量与冻储 6周和 8周的同种异体移植气管相同 ,但在第 2周时大部分黏膜呈现灰黑色 ,部分软骨环外露。大部分移植气管在术后短期内坏死。显微镜下见移植气管内有大量单核细胞浸润 ,病理变化尤以未冻储、冻储 1d和 2周组为重。而冻储 6周和 8周的气管移植后与自体移植的气管无明显差别 ,未见有单核细胞浸润 ,可长期存活。结论　气管经深低温冻储 6周以上才可以消减其抗原性 ,同种异体移植后不发生免疫排斥反应 。Objective To investigate the proper time of cryo\|preserving tracheal allograft so as to minimize its antigenicity. Methods\ On a dog model, this study was carried out by allografting a tracheal into a muscular flap formed with sternocephalic muscle and sternohyoid-sternothyroid muscle. The tracheal was treated with cryopreservation in defferent intervals. The viability of the graft was evaluated by the examination of fiberoptic bronchoscopy, histopathology and microangiography.The blood flow of the tracheal mucous was measured with a blood flowmeter and the survival area was decided in the calculation of the percentage. Results\ There are no significant differences in the mucous membrane appearance and the mucosal blood flow one week after the surgery among the non\|cryopreservation group and the groups treated with cryopreservation in 1 day, 2 weeks,4 weeks,6 weeks and 8 weeks. The graft was found to start necrosis 2 weeks after the transplantation with the infiltration of mononuclear cells examined under light microscope in almost all of the groups, especially in the non\|cryopreservation group and the groups treated with cryopreservation in 1 day, 2 weeks. However, there was no significant difference among the autograft group and the allograft groups cryopreservated in 6 weeks and 8 weeks, and the infiltration of the mononuclear cells was not found in these groups either. Conclusion\ The antigenicity of the tracheal allografts could be significantly decreased by the treatment of cryopreservation over 6 weeks.

关 键 词：深低温 气管抗原 同种异体移植 冷冻 免疫抑制剂 

分 类 号：R653[医药卫生—外科学]