人参茎叶皂甙增强糖皮质激素受体转录激活效应的实验研究  

Experimantal Study on the Effect of Ginsenosides of Ginseng Stem and Leaf in Enhancing the Transactivation of Glucocorticoid Receptor Induced by Dexamethasone in Vitro

作　　者：李勇[1] 李敏[2] 王喜[1] 胡侠[1] 凌昌全[1] 

机构地区：[1]第二军医大学附属长海医院中医科,上海200433 [2]第二军医大学海军医学系

出　　处：《中国中西医结合杂志》2004年第8期710-713,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家杰出青年基金资助项目(No .30 0 2 50 4 5);国家自然科学基金资助项目(No.39870 92 0 )

摘　　要：目的探讨人参茎叶皂甙 (ginsenosides stem leaves ,GSL)对糖皮质激素受体 (glucocorticoidre ceptor,GR)转录激活效应的影响。 方法将糖皮质激素受体荧光素酶报告基因 pGRE tk Luc与内参照基因pRL SV4 0共同瞬时转染细胞 ,利用双荧光素酶报告基因检测方法 (Dual LuciferaseReporterAssay) ,观察地塞米松 (dexamethasone ,Dex)和GSL对报告基因表达的影响。结果Dex诱导HL770 2细胞内报告基因的表达呈剂量和时间依赖性 ,Dex的最大诱导倍数达 93倍。糖皮质激素受体的拮抗剂RU4 86可以阻断Dex对报告基因的诱导 ;GSL不能诱导报告基因的表达 ,但可使Dex对GR报告基因的诱导表达效应提高6 1 4 %。Objective To investigate the effect of ginsenosides of ginseng stem and leaf (GSL) on the transcription activity of glucocorticoid receptor (GR). Methods HL7702 cells were transiently cotransfected by GR luciferase reporter plasmid pGRE tk Luc and inner referential gene pRL SV40 plasmid, and the effects of dexamethasone (Dex) and GSL on the reporter gene expression were detected by dual luciferase reporter assay. Results The reporter gene expression Dex induced in HL7702 was dose and time dependent, it could be multiplied to 93 times in maximum, and could be blocked by the GR antagonist RU486. GSL could not induce the expression of reporter gene but could elevate the induction of Dex for 61 4%. Conclusion GSL could enhance the inducing effect of Dex on transcription activity of GR in vitro.

关 键 词：人参茎叶皂甙 糖皮质激素 受体转录激活效应 GSL 拮抗剂 

分 类 号：R285[医药卫生—中药学]