血清中硫脂质的提纯及其结构分析  

Purification and Structural Characterization of Sulfated Lipids in Human Sera

作　　者：林蓓[1] 张淑兰[1] 岩森正男[2] 

机构地区：[1]中国医科大学第二附属医院妇产科,沈阳110004 [2]日本近畿大学理工学部化学科

出　　处：《分析科学学报》2004年第5期525-527,共3页Journal of Analytical Science

摘　　要：应用离子交换及硅胶柱色谱法分离提纯血清中酸性脂质,并采用薄层色谱法(TLC)、TLC免疫染色法、质谱法及溶化分解等方法分析血清中硫脂质的种类及结构。实验确定了样品的提纯体系,证实胆固醇硫酸及脑硫脂是血清中的主要硫脂质。The sulfated lipids, cholesterol sulfate (CS) and sulfatide, were firstly separated from human sera by ion exchange and silica gel column chromatographies. Their structures were characterized by TLC, TLC-immunostaining, negative-ion fast atom bombardment mass spectrometry and solvolysis. The results revealed that CS and sulfatide were the main sulfated lipids in human sera and their quantitativ determination in a nanomolar scale was readily perfomed by TLC.

关 键 词：血清 硫脂质 提纯 结构 离子交换 硅胶柱色谱法 胆固醇硫酸 脑硫脂 

分 类 号：Q592.1[生物学—生物化学]