传染性法氏囊病病毒河北分离株(HB-bp)基因组A节段全长cDNA的克隆和序列分析  

Cloning and sequence analysis of full-length segment A of an infectious bursal disease virus(HB-bp) strain

作　　者：倪风娥[1] 秦建华[1] 赵月兰[2] 张宁[2] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]河北省畜牧兽医研究所

出　　处：《河北农业大学学报》2004年第5期84-87,92,共5页Journal of Hebei Agricultural University

摘　　要：采用RT-PCR技术,以传染性法氏囊病病毒河北分离株(HB-bp)基因组RNA为模板,扩增并克隆了IBDVHB-bp株基因组A节段全长cDNA。结果表明:克隆的A节段全长共3142个核苷酸,包括5′、3′端非编码区(NCRs)和2个部分重叠的开放阅读框(ORF1和ORF2),与其它血清Ⅰ型毒株相比,HB-bp株在核苷酸水平上有32～146个核苷酸的差异,同源性为95 4%～98 8%;在氨基酸水平上有5～35个氨基酸的替代,同源性为96 9%～99 5%,与血清Ⅱ型毒株同源性为89 8%～90 8%。与其他超强毒株的同源性最高,具有超强毒株的特征性氨基酸,因此HB-bp株为中国的一个超强毒株。Sequence and phylogenetic analysis of segement A of Chinese infectious bursal disease virus (IBDV) HB-bp strain isolated from Hebei Province was studied in this thesis. The results show that the cloned 3142bp contains two overlapping ORFs.The large ORF of 3036bp coding a polyprotein(VP2-4-3),whereas the small ORF is 435bp coding VP5.The deduced amino acid sequences HB-bp strain were compared with those of other serotypeⅠand serotypeⅡstrains.The results show that the sequence of HB-bp segement A shared 95.4%～98.8 % homology with other serotype Ⅰand 89.8%～90.8% homology with other serotype Ⅱat nucleotide level. The HB-bp was closly related to vvIBDV with others . The specific sequence of vvIBDV were found from VP2,VP4 and VP3 regions.The result shows that a vvIBDV strain was obtained.

关 键 词：传染性法氏囊病病毒 基因组 克隆 序列分析 

分 类 号：S852.65[农业科学—基础兽医学] Q78[农业科学—兽医学]